Overview

  • Product name
    Anti-NOP2 antibody [22G1]
    See all NOP2 primary antibodies
  • Description
    Mouse monoclonal [22G1] to NOP2
  • Specificity
    Specificity was determined by western blot analysis of extracts from a yeast strain expressing Nop2p under GAL-UAS control and from strains containing nop2 ts alleles that express Nop2p isoforms of altered molecular weight.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    This was raised against a yeast nucleolar prep, and screened by immunocytochemistry and western blotting.

  • General notes
    Nop2p is the yeast homologue of the human p120 nucleolar "proliferation" antigen.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 10mM Sodium Azide
    Constituents: Tissue Culture Supernatant
  • Purity
    Tissue culture supernatant
  • Purification notes
    Sterile filtered.
  • Clonality
    Monoclonal
  • Clone number
    22G1
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab4638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notes
    WB: 1/10000 (cell lysates) - 1/25000 (nuclear fractions). For other (non-ECL) detection methods, 1/1000 - 1/5000. Predicted molecular weight: 100 kDa.
    IF: 1/500 - 1/5000 (yeast cells).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      NOP2 belongs to the methyltransferase superfamily of the RsmB/NOP family. NOP2 may play a role in the regulation of the cell cycle and the increased nucleolar activity that is associated with the cell proliferation. May act as ribosomal RNA methyltransferase.
    • Cellular localization
      Nuclear; nucleolus
    • Alternative names
      • NSUN2 antibody
      • MISU antibody
      • NOL1 antibody
      • NOL1/NOP2/Sun domain family, member 1 antibody
      • NOP120 antibody
      • NOP2 nucleolar protein homolog yeast antibody
      • NOP2/Sun domain family, member 1 antibody
      • NSUN1 antibody
      • Nucleolar antigen p120 antibody
      • Nucleolar protein 1 120kDa antibody
      • Nucleolar protein 1 antibody
      • Nucleolar protein 2 homolog antibody
      • Nucleolar protein 2 homolog yeast antibody
      • p120 antibody
      • Proliferating cell nucleolar antigen p120 antibody
      • Proliferating cell nucleolar protein p120 antibody
      • Proliferation associated nucleolar protein p120 antibody
      • putative ribosomal RNA methyltransferase NOP2 antibody
      • SAKI antibody
      • TRM4 antibody
      see all

    References

    ab4638 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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