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This was raised against a yeast nucleolar prep, and screened by immunocytochemistry and western blotting.
Our Abpromise guarantee covers the use of ab4640 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/1000 - 1/10000.
|IP||Use at an assay dependent concentration.
Use 2-4 µl with the amount of lysate derived from 5 OD600 units of yeast culture.
ab4640 has not yet been referenced specifically in any publications.