Overview

  • Product nameAnti-Noradrenaline antibody
  • Description
    Rabbit polyclonal to Noradrenaline
  • Specificityab8887 targets conjugated noradrenaline only it does not recognize free noradrenaline.
  • Tested applicationsSuitable for: ICC, IHC-Fr, IHC-Pmore details
  • Immunogen

    Chemical/ Small Molecule corresponding to Noradrenaline conjugated to Bovine Serum Albumin (BSA).

Properties

Applications

Our Abpromise guarantee covers the use of ab8887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
IHC-Fr
IHC-P
  • Application notesELISA: Use at an assay dependent concentration.
    ICC: Use at an assay dependent concentration.
    IHC-P: Use at an assay dependent dilution (PMID 18457899).
    IHC-Fr: Use at an assay dependent concentration.

    The recommended dilution can be between 1:2,000-1:5,000.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceNoradrenaline (NA) is a catecholamine neurotransmitter at postganglionic sympathetic fibres, produced following a series of steps from the essential amino acid phenylalanine. Terminal postganglionic sympathetic fibres have varicosities (where NA is synthesised and stored) which form synapses with the effector organ. The adrenal medulla is a major source of catecholamines (such as NA and adrenaline), however, most of the NA produced in the adrenal medulla is converted into adrenaline. NA is key in the fight-or-flight response and also directly increases heart rate, energy release from fat, and muscle readiness.
    • Cellular localizationSecreted
    • Alternative names
      • Arterenol antibody
      • NA antibody
      • Norepinephrine antibody

    Anti-Noradrenaline antibody images

    • ab8887 staining Noradrenaline (red) in rat smooth muscle cells from the aorta.

      Cells were transfected with conjugated Noradrenaline before being fixed and permeabilized with 0.1% Triton X-100 for 20 minutes. Samples were incubated with primary antibody at 1/500 dilution. A fluorescence-conjugated anti-rabbit IgG was used as the secondary antibody.

    References for Anti-Noradrenaline antibody (ab8887)

    This product has been referenced in:
    • Kondo Y  et al. Expression and Role of the BDNF Receptor-TrkB in Rat Adrenal Gland under Acute Immobilization Stress. Acta Histochem Cytochem 43:139-47 (2010). ICC/IF . Read more (PubMed: 21245980) »
    • Johnson KB  et al. Modification of proteins by norepinephrine is important for vascular contraction. Front Physiol 1:131 (2010). WB, ICC/IF, IHC-P . Read more (PubMed: 21423373) »

    See all 4 Publications for this product

    Product Wall

    Thank you for your enquiry and interest in our products.

    The storage buffer of this antibody only contains Glycerol which is nonhazardous, there is no MSDS for ab8887. A general SDS document can be downloaded from the on-line datasheet.
    ...

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    Thank you very much for your reply.

    For ab6454, it looks like the antibody was tested with glutaraldehyde-perfused tissue sections. There is a link to "Immunohisto and cytochemistry" protocol at the very bottom of the datasheet- Read More

    Thank you very much for contacting us with your enquiry.

    I apologize for the confusion regarding these products. Both ab8887 and ab6454 are antibodies that target conjugated noradrenaline, instead of free noradrenaline. As a small molecule, N...

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    Thank you for contacting Abcam.


    Attached please find the MSDS you requested.


    Please do not hesitate to contact me if you have any additional questions.

    Thank you for contacting Abcam regarding ab8887.

    I have confirmed with the laboratory that unfortunately, there is no concentration information available for this antibody.

    I apologize for any inconvenience this may cause. Please ...

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    Thank you for your enquiry. The FAQ you noticed is from Thursday 25-November-2004 at which time the antibody had not been tested in IHC-P. We since have data from a publication that came to our attention in Sept 2008. PMID 18457899. Thi...

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    Thank you for your enquiry. Regarding "using protein in the phenol-ethanol supernatant for ELISA", would you first precipitate the protein? In either case, I'm not sure if the protein would be recognized by the antibodies since we have not tested t...

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    Thank you for your patience and forwarding your detailed protocol on-line. We are very sorry to hear that you are having some problem with this antibody. Looking through your email, I can understand that you have fixed the tissue sections in parafor...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"