The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at an assay dependent concentration.
ICC: Use at an assay dependent concentration.
IHC-P: Use at an assay dependent dilution (PMID 18457899).
IHC-Fr: Use at an assay dependent concentration.
The recommended dilution can be between 1:2,000-1:5,000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceNoradrenaline (NA) is a catecholamine neurotransmitter at postganglionic sympathetic fibres, produced following a series of steps from the essential amino acid phenylalanine. Terminal postganglionic sympathetic fibres have varicosities (where NA is synthesised and stored) which form synapses with the effector organ. The adrenal medulla is a major source of catecholamines (such as NA and adrenaline), however, most of the NA produced in the adrenal medulla is converted into adrenaline. NA is key in the fight-or-flight response and also directly increases heart rate, energy release from fat, and muscle readiness.
Anti-Noradrenaline antibody images
Immunocytochemistry/ Immunofluorescence - Noradrenaline (conjugated) antibody (ab8887)Image from Johnson KB et al., Front Physiol. 2010 Oct 4;1:131. Fig 4.; doi: 10.3389/fphys.2010.00131; 04 October 2010, Front. Physio. 1:131.
ab8887 staining Noradrenaline (red) in rat smooth muscle cells from the aorta.
Cells were transfected with conjugated Noradrenaline before being fixed and permeabilized with 0.1% Triton X-100 for 20 minutes. Samples were incubated with primary antibody at 1/500 dilution. A fluorescence-conjugated anti-rabbit IgG was used as the secondary antibody.
References for Anti-Noradrenaline antibody (ab8887)
This product has been referenced in:
Kondo Y et al. Expression and Role of the BDNF Receptor-TrkB in Rat Adrenal Gland under Acute Immobilization Stress. Acta Histochem Cytochem43:139-47 (2010).
Read more (PubMed: 21245980) »
Johnson KB et al. Modification of proteins by norepinephrine is important for vascular contraction. Front Physiol1:131 (2010).
WB, ICC/IF, IHC-P
Read more (PubMed: 21423373) »