Anti-Noradrenaline transporter antibody (ab41559)

Overview

  • Product nameAnti-Noradrenaline transporter antibodySee all Noradrenaline transporter primary antibodies ...
  • Description
    Rabbit polyclonal to Noradrenaline transporter
  • SpecificityAb41559 does not cross react with dopamine and serotonin transporters.
  • Tested applicationsELISA, Dot Blot, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    The noradrenaline transporter antibodies were generated against unique N-terminal peptides that are unique to the noradrenaline transporter protein. The antibodies are raised utilizing linear, multiple antigenic peptides or cyclic peptide methodology.

  • Positive control
    • Rat brain cortical membranes.

Properties

Applications

Our Abpromise guarantee covers the use of ab41559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/4000.
Dot Blot 1/4000.
WB 1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 70 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionAmine transporter. Terminates the action of noradrenaline by its high affinity sodium-dependent reuptake into presynaptic terminals.
  • Involvement in diseaseOrthostatic intolerance
  • Sequence similaritiesBelongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A2 subfamily.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • NAT1 antibody
    • NET antibody
    • NET antibody
    • NET1 antibody
    • Neurotransmitter transporter antibody
    • Norepinephrine transporter antibody
    • SC6A2_HUMAN antibody
    • SLC6A2 antibody
    • SLC6A5 antibody
    • SLC6A5 antibody
    • Sodium dependent noradrenaline transporter antibody
    • Sodium-dependent noradrenaline transporter antibody
    • Solute carrier family 6 (neurotransmitter transporter norepinephrine) member 5 antibody
    • Solute carrier family 6 (neurotransmitter transporter, noradrenalin), member 2 antibody
    • Solute carrier family 6 member 2 antibody
    • Solute carrier family 6 member 2 antibody
    • Solute carrier family 6 member 5 antibody
    see all

Anti-Noradrenaline transporter antibody images

  • Anti-Noradrenaline transporter antibody (ab41559) at 1/250 dilution + rat brain cortical membrane cell lysate at 70 µg

    Predicted band size : 70 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 28 kDa,32 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab41559 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41559, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Noradrenaline transporter antibody (ab41559)

ab41559 has not yet been referenced specifically in any publications.

Product Wall

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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Thank you for your email.

Customer has mentioned in previous mail that perfusion fixed sections were used which actually corresponds to IHC-FoFr not ICC/IF so I have ignored ICC/IF.

Do you think customer have also used the antibody ...

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Many thanks for providing details.

I have thoroughly checked the details again. We unfortunately haven't yet tested this antibody in Immunohistochemistry (IHC-FoFR) so I am sorry we are not sure how well the antibody will perform. Although yo...

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Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further questions for better understanding of the problem;

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Thank you for your patience. For the antibody ab41559 the lab used the following WB procedure: Dissected tissue homogenized in RIPA buffer with protease inhibitors.  The RIPA buffer will extract all integral membrane proteins in partially denatured...

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Thank you for your enquiry. On the antibody datasheet it was stated IM; immunomicroscopy. This was actually a mistake. Please accept my apolagies, it has been removed from the datasheet. This antibody has not been tested for use by immunohistochemi...

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