Overview

  • Product nameAnti-Noradrenaline transporter antibody
    See all Noradrenaline transporter primary antibodies
  • Description
    Rabbit polyclonal to Noradrenaline transporter
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal amino acids 396 - 445 (STLSGSTFWA VVFFVMLLAL GLDSSMGGME AVITGLADDF QVLKRHRKLF) of Human Noradrenaline transporter (NP_001034)

  • Positive control
    • Human fetal heart lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab84057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 70 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionAmine transporter. Terminates the action of noradrenaline by its high affinity sodium-dependent reuptake into presynaptic terminals.
  • Involvement in diseaseOrthostatic intolerance
  • Sequence similaritiesBelongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A2 subfamily.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • NAT1 antibody
    • NET antibody
    • NET1 antibody
    • Neurotransmitter transporter antibody
    • Norepinephrine transporter antibody
    • SC6A2_HUMAN antibody
    • Slc6a2 antibody
    • SLC6A5 antibody
    • Sodium dependent noradrenaline transporter antibody
    • Sodium-dependent noradrenaline transporter antibody
    • Solute carrier family 6 (neurotransmitter transporter norepinephrine) member 5 antibody
    • Solute carrier family 6 (neurotransmitter transporter, noradrenalin), member 2 antibody
    • Solute carrier family 6 member 2 antibody
    • Solute carrier family 6 member 5 antibody
    see all

Anti-Noradrenaline transporter antibody images



  • Predicted band size : 70 kDa
    Western blot analysis of Human fetal heart tissue lysate labeling Noradrenaline transpoter with ab84057 at 1.0µg/ml.


  • Predicted band size : 70 kDa
    Western blot analysis of Human adult placenta tissue lysate labeling Noradrenaline transpoter with ab84057 at 1.0µg/ml.
  • Anti-Noradrenaline transporter antibody (ab84057) at 1 µg/ml + Human fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 70 kDa
    Observed band size : 64 kDa (why is the actual band size different from the predicted?)
    Gel concentration 12%
  • ICC/IF image of ab84057 stained SKNSH cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84057, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Noradrenaline transporter antibody (ab84057)

This product has been referenced in:
  • Bayles R  et al. Epigenetic modification of the norepinephrine transporter gene in postural tachycardia syndrome. Arterioscler Thromb Vasc Biol 32:1910-6 (2012). Read more (PubMed: 22723437) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"