• Product name
  • Description
    Rabbit polyclonal to NORE1
  • Specificity
    ab22770 primarily detects NORE1 protein (isoform A) at ~50kDa in human HEK293 cells and also in mouse brain lysate. This antibody is also expected to detect a number of NORE1 isoforms based on an immunogen BLAST search. In human HEK293 cells, ab22770 faintly detects the following NORE1, RASSF3 and RASSF5 isoforms (Swiss Prot ID in brackets): 44kDa (Q5SY32), 39kDa (Q59GG4), 37kDa (Q8WWV9), 31kDa (Q8WXF4) and 21kDa (Q8TEK8) bands. In mouse brain ab22770 also faintly detects NORE1 isoform B at ~32kDa band (Q8C2E8).
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human NORE1.

    (Peptide available as ab23380.)

  • Positive control
    • HEK293 cell lysate, Mouse brain lysate



Our Abpromise guarantee covers the use of ab22770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).


  • Function
    Potential tumor suppressor. Seems to be involved in lymphocyte adhesion by linking RAP1A activation upon T cell receptor or chemokine stimulation to integrin activation. Isoform 2 stimulates lymphocyte polarization and the patch-like distribution of ITGAL/LFA-1, resulting in an enhanced adhesion to ICAM1. Together with RAP1A may participate in regulation of microtubule growth. The association of isoform 2 with activated RAP1A is required for directional movement of endothelial cells during wound healing. May be involved in regulation of Ras apoptotic function. The RASSF5-STK4 complex may mediate HRAS1 and KRAS induced apoptosis.
  • Tissue specificity
    Widely expressed. Frequently down-regulated in lung tumor cell lines and primary lung tumors.
  • Sequence similarities
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 Ras-associating domain.
    Contains 1 SARAH domain.
  • Cellular localization
    Cytoplasm. Cytoplasm > cytoskeleton. Isoform 2 is mainly located in the perinuclear region of unstimulated primary T cells. Upon stimulation translocates to the leading edge and colocalizes with ITGAL/LFA-1 in the peripheral zone of the immunological synapse. Isoform 2 is localized to growing microtubules in vascular endothelial cells and is dissociated from microtubules by activated RAP1A.
  • Information by UniProt
  • Database links
  • Alternative names
    • Maxp 1 antibody
    • MAXP1 antibody
    • MGC10823 antibody
    • MGC17344 antibody
    • New ras effector 1 antibody
    • NORE 1 antibody
    • NORE1A antibody
    • NORE1B antibody
    • Novel Ras effector 1 antibody
    • RAP1 Binding Protein antibody
    • RAPL antibody
    • Ras association (RalGDS/AF 6) domain family 5 antibody
    • Ras association (RalGDS/AF 6) domain family member 5 antibody
    • Ras association domain containing family protein 5 antibody
    • Ras association domain-containing protein 5 antibody
    • Ras effector like protein antibody
    • RASF5_HUMAN antibody
    • RASSF 3 antibody
    • RASSF 5 antibody
    • RASSF3 antibody
    • RASSF5 antibody
    • Regulator for cell adhesion and polarization enriched in lymphoid tissue antibody
    • Regulator for cell adhesion and polarization enriched in lymphoid tissues antibody
    • Tumor suppressor RASSF3 antibody
    see all


  • All lanes : Anti-NORE1 antibody (ab22770) at 1 µg/ml

    Lane 1 : HEK293 cell lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : HEK293 cell lysate with Human NORE1 peptide (ab23380) at 1 µg/ml
    Lane 4 : Mouse brain lysate with Human NORE1 peptide (ab23380) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Alexa fluor goat polyclonal to rabbit IgG at 1/10000 dilution

    Predicted band size : 47 kDa
    Observed band size : ~50 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab22770 stained human Hek293 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab22770, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • IHC image of ab22770 staining NORE1 in Human Tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab22770, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


ab22770 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us.
I am sorry to hear that the antibody is not working as expected on IHC-P sections.

Could you please send me more details about the conditions that were tried by your colleague, such as antibody dilution and i...

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Thank you for your enquiry. We do have a GABARAPL2 antibody (ab15858) in addition to three NORE1 antibodies (ab22770,ab33292,ab16757), I am in the process of contacting the lab to determine whether any of our NORE1 antibodies can detect RAPL as wel...

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