Anti-Norovirus Capsid protein VP1 antibody (ab92976)


  • Product name
    Anti-Norovirus Capsid protein VP1 antibody
  • Description
    Rabbit polyclonal to Norovirus Capsid protein VP1
  • Host species
  • Specificity
    Species Reactivity: Norovirus Hu/Texas/TCH04-577/2004/US
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Other
  • Immunogen

    Synthetic peptide corresponding to N terminal residues of Norovirus Capsid protein VP1



Our Abpromise guarantee covers the use of ab92976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa. for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA 1/2000 - 1/5000.
ICC/IF 1/100.


  • Relevance
    Norovirus is an RNA virus of the Caliciviridae taxonomic family, causes approximately 90% of epidemic non bacterial outbreaks of gastroenteritis around the world and is responsible for 50% of all foodborne outbreaks of gastroenteritis in the US. Norovirus affects people of all ages. The viruses are transmitted by faecally contaminated food or water and by person to person contact. Noroviruses contain a positive-sense RNA genome of approximately 7.5 kbp, encoding a major structural protein (VP1) of about 58~60 kDa and a minor capsid protein (VP2). The virus particles demonstrate an amorphous surface structure when visualized using electron microscopy and are between 27-38 nm in size. Capsid protein VP1 attaches virion to target cells by binding histo-blood group antigens present on gastroduodenal epithelial cells. Soluble capsid protein may play a role in viral immunoevasion. Capsid protein VP1 binds to histoblood group antigens at surface of target cells. The shell domain (S domain) contains elements essential for the formation of the icosahedron. The Protruding domain (P domain) is divided into subdomains P1 and P2. P domain interacts in dimeric contacts that increase the stability of the capsid and form the protrusions on the virion. An hypervariable region in P2 is thought to play an important role in receptor binding and immune reactivity.
  • Database links
  • Alternative names
    • VP1 antibody


  • Ab92976 staining Norovirus Capsid protein VP1 in RAW 264.7 cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Digitonin (50µg/ml) and blocked with 1% serum for 1 hour at 21°C. Samples were incubated with primary antibody (1/100 dilution) for 1 hour at 21°C. An Alexa Fluor® 488 Donkey Anti-Mouse was used as the secondary antibody at 1/1000 dilution.

    See Abreview


This product has been referenced in:
  • Zhang Y  et al. Antiviral activity of shikonin ester derivative PMM-034 against enterovirus 71 in vitro. Braz J Med Biol Res 50:e6586 (2017). Read more (PubMed: 28832767) »
  • Enosi Tuipulotu D  et al. RNA Sequencing of Murine Norovirus-Infected Cells Reveals Transcriptional Alteration of Genes Important to Viral Recognition and Antigen Presentation. Front Immunol 8:959 (2017). Read more (PubMed: 28848558) »

See all 5 Publications for this product

Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence
Mouse Cell (RAW 264.7 (murine macrophage))
Yes - Digitonin 50ug/ml
RAW 264.7 (murine macrophage)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Aug 10 2017

Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (8%)
Mouse Cell lysate - whole cell (macrophage cell line)
macrophage cell line
MNV infection
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Oct 29 2014

Thank you for contacting us.

The antibodies have only been tested in ELISA. We do not have any data for western blotting.

Several of these are monoclonal antibodies that are raised against various strains of Norovirus, and the epi...

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Thank you for your inquiry.
I translated your sequence into the following protein sequence:

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Thank you for your reply. There is about a 74% similarity between your sequence the immunogen sequence that was used for ab92976. Normally we look for about an 85% similarity before recommending an antibody be used on an untested species. If you will s...

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Unfortunately I cannot determine if the antibody will react with the strain that you are working on. If you could send me the sequence of your particular strain then I could determine if there is a significant match between that and the peptide sequenc...

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