Overview

  • Product name
  • Description
    Rabbit polyclonal to NOSIP
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant protein fragment contain a sequence corresponding to a region within amino acids 17-270 of Human NOSIP (AAH09299).

  • Positive control
    • 293T, A431, Raji whole cell lysate; U87 xenograft

Properties

Applications

Our Abpromise guarantee covers the use of ab102087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH 8.0)
WB 1/500 - 1/3000. Predicted molecular weight: 33 kDa.

Target

  • Relevance
    NOSIP is a novel type of modulator that promotes translocation of eNOS from the plasma membrane to intracellular sites, thereby uncoupling eNOS from plasma membrane caveolae and inhibiting NO synthesis.
  • Cellular localization
    Cytoplasm. Nucleus. Note: Translocates from nucleus to cytoplasm in the G2 phase of the cell cycle.
  • Database links
  • Alternative names
    • CGI 25 antibody
    • CGI25 antibody
    • CGI25 protein antibody
    • eNOS interacting protein antibody
    • Nitric oxide synthase interacting protein antibody
    see all

Images

  • All lanes : Anti-NOSIP antibody (ab102087) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 33 kDa
  • Immunohistochemical analysis of paraffin-embedded U87 xenograft, using ab102087 at 1/100 dilution.

References

This product has been referenced in:
  • Gong ZY  et al. Glutamine with probiotics attenuates intestinal inflammation and oxidative stress in a rat burn injury model through altered iNOS gene aberrant methylation. Am J Transl Res 9:2535-2547 (2017). Read more (PubMed: 28560003) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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