Notch 2 antibody (ab8926)

Product Name 

Notch 2 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Notch 2

Immunogen 

Synthetic peptide: CRDASNHKRREPVGQD, corresponding to amino acids 1719-1733 of Human Notch 2.This is the N-terminal sequence of the cleaved N intracellular domain (NICD). A residue of cysteine was added to the amino terminal end to facilitate coupling (Peptide available as ab1389.)
BLAST 'CRDASNHKRREPVGQD' with or

Reacts with 

Hu

Predicted to react 
(species key)

Ms, Rat
(Due to sequence homology)

Epitope 

The epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form.

Tested applications 
(see key)

ELISA, ICC/IF, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/30'000 - 1/90'000.
ICC/IF: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cell membrane; Single-pass type I membrane protein. Notch 2 intracellular domain: Nucleus (following proteolytical processing NICD is translocated to the nucleus)

Research areas 

Stem Cells >> Neural Stem Cells >> Surface Molecules
Stem Cells >> Neural Stem Cells >> Intracellular
Stem Cells >> Signaling Pathways >> Notch >> Nuclear
Stem Cells >> Signaling Pathways >> Notch >> Surface Molecules
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Neurology process >> Notch Pathway

Relevance 

Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

serum with 0.02% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

70.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for Notch 2 antibody images, references, products related to ab8926 and other tools.

Notch 2 antibody images:

References for Notch 2 antibody (ab8926)

  This product has been used in: (two most recent references)

Saravanamuthu SS et al. Notch signaling is required for lateral induction of Jagged1 during FGF-induced lens fiber differentiation. Dev Biol 332:166-76 (2009). ICC/IF; Rat. PubMed: 19481073

Campa VM et al. Notch activates cell cycle reentry and progression in quiescent cardiomyocytes. J Cell Biol 183:129-41 (2008). ICC/IF; Rat. PubMed: 18838555

See all 3 publication references for this product.


If you publish research using ab8926 please let us know so that we can cite the reference on this datasheet.

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