Anti-Notch 2 intracellular domain antibody (ab8927)

Overview

  • Product nameAnti-Notch 2 intracellular domain antibody
  • Description
    Rabbit polyclonal to Notch 2 intracellular domain
  • SpecificityWhen tested on human cells overexpressing active Notch 2, this antibody detects a band at about 110kD that represents active Notch 2. This band is not seen in the control cells, confirming that it is Notch 2. The intracellular domain of Notch 2 has a predicted band size of 110kD, corresponding to this band. However, multiple other non-specific bands were also seen in the same experiment.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Peptide:

    C-NAAERTPSHSGHLQ

    aa 2396-2409 (the total protein is 2471 aa).

  • General notes


    Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
  • PurityWhole antiserum
  • Primary antibody notesSynthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/400. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).

Target

  • RelevanceSynthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.
  • Cellular localizationCell membrane; Single-pass type I membrane protein. NICD: Nucleus. Note=Following proteolytical processing NICD is translocated to the nucleus.
  • Database links
  • Alternative names
    • AGS2 antibody
    • hN2 antibody
    • Notch homolog 2 antibody
    • Notch2 antibody

Anti-Notch 2 intracellular domain antibody images



  • Predicted band size : 110 kDa


    Western Blot analysis for Notch 2 expression performed on 100µg of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constituvely active Notch 2 (intra cellular Notch 2).

    Lanes1: 24 hours after transfection. 
    Lane 2: 48 hours after transfection.
    Lane 3: 72 hours after transfection.
    Lane 4: Control cells 24 hours after transfection.
    Lane 5: Control cells 48 hours after transfection.
    Lane 6: Control cells 72 hours after transfection.

    The band at about 110kD represents active Notch 2. This band is not seen in the control cells, confirming that it is Notch 2. The intracellular domain of Notch 2 has a predicted band size of 110kD, corresponding to this band.

    Protein cell lysates were run on a 10% SDS-page gel, blotted onto Hybond C membrane, blocked overnight in PBS-Tween 20 supplemented with 5% Non-fat Milk and hybridized with ab8927 rabbit polyclonal antibody specific for Notch 2 at a 1:400 dilution.

    ECL was used as visualization method.

References for Anti-Notch 2 intracellular domain antibody (ab8927)

This product has been referenced in:
  • Chen CD  et al. Visualization of APP dimerization and APP-Notch2 heterodimerization in living cells using bimolecular fluorescence complementation. J Neurochem 97:30-43 (2006). Read more (PubMed: 16515557) »

See 1 Publication for this product

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