Anti-Notch1 intracellular domain antibody (ab83232)


  • Product name
    Anti-Notch1 intracellular domain antibody
    See all Notch1 intracellular domain primary antibodies
  • Description
    Rabbit polyclonal to Notch1 intracellular domain
  • Tested applications
    Suitable for: WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Human, Apteronotus leptorhynchus
    Predicted to work with: Rat, Horse, Chicken, Guinea pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within c-terminal amino acids 2506-2555 (EHPFLTPSPE SPDQWSSSSP HSNVSDWSEG VSSPPTSMQS QIARIPEAFK) of Human Notch1 intracellular domain (NP_060087).

  • Positive control
    • 293T cell lysate.



Our Abpromise guarantee covers the use of ab83232 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 273 kDa for Notch1 full length protein and 88 kDa for Notch1 intracellular domain. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-Fr Use at an assay dependent concentration.


  • Function
    Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia.
  • Tissue specificity
    In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.
  • Involvement in disease
    Defects in NOTCH1 are a cause of bicuspid aortic valve (BAV) [MIM:109730]. A common defect in the aortic valve in which two rather than three leaflets are present. It is often associated with aortic valve calcification and insufficiency. In extreme cases, the blood flow may be so restricted that the left ventricle fails to grow, resulting in hypoplastic left heart syndrome.
  • Sequence similarities
    Belongs to the NOTCH family.
    Contains 5 ANK repeats.
    Contains 36 EGF-like domains.
    Contains 3 LNR (Lin/Notch) repeats.
  • Post-translational
    Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
    O-glycosylated on the EGF-like domains. Contains both O-linked fucose and O-linked glucose.
    Ubiquitinated; undergoes 'Lys-29'-linked polyubiquitination catalyzed by ITCH.
  • Cellular localization
    Cell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • hN1 antibody
    • Neurogenic locus Notch homolog protein 1 antibody
    • NICD antibody
    • NOTC1_HUMAN antibody
    • Notch 1 antibody
    • Notch 1 intracellular domain antibody
    • Notch homolog 1 translocation associated antibody
    • notch1 antibody
    • TAN1 antibody
    • Translocation associated Notch protein TAN1 antibody
    • Translocation-associated notch protein TAN-1 antibody
    see all

Anti-Notch1 intracellular domain antibody images

  • Anti-Notch1 intracellular domain antibody (ab83232) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Observed band size : 88 kDa (why is the actual band size different from the predicted?)
  • ab83232 staining Notch1 intracellular domain in 16µm sections of Apteronotus leptorhynchus brain tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in paraformaldehyde, permeabilized using 0.3% Triton X-100, then blocked with 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS for 1 hour at 24°C, and then incubated with ab83232 at a 1/100 dilution for 18 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal, used at a 1/200 dilution.

    See Abreview

References for Anti-Notch1 intracellular domain antibody (ab83232)

This product has been referenced in:
  • Saha SK  et al. KRT19 directly interacts with ß-catenin/RAC1 complex to regulate NUMB-dependent NOTCH signaling pathway and breast cancer properties. Oncogene 36:332-349 (2017). WB ; Human . Read more (PubMed: 27345400) »
  • Wang T  et al. Fibulin-4 is associated with prognosis of endometrial cancer patients and inhibits cancer cell invasion and metastasis via Wnt/ß-catenin signaling pathway. Oncotarget 8:18991-19012 (2017). WB ; Human . Read more (PubMed: 28177909) »

See all 3 Publications for this product

Product Wall

Western blot
Rat Cell lysate - whole cell (neonatal rat primary cardiomyocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
40nm Triptolide for 24hrs
neonatal rat primary cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 15°C

Verified customer

Submitted Jun 20 2017

Immunohistochemistry (PFA perfusion fixed frozen sections)
Mouse Tissue sections (Tongue epithelium)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate buffer followed by Trypsin
Tongue epithelium
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jun 13 2016

IHC - Wholemount
Zebrafish Embryo (48hpf whole embryos)
48hpf whole embryos

Abcam user community

Verified customer

Submitted Feb 09 2015

Western blot
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Sep 28 2012

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for contacting us. I am sorry that antibody is not working as expected.

As we discussed on phone please try the troubleshooting steps and let me know how the antibodyperform.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Frozen sections)
Apteronotus leptorhynchus Tissue sections (Brain)
Yes - 0.3% Triton X-100
Blocking step
3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C

Dr. Ruxandra Sirbulescu

Verified customer

Submitted Nov 28 2011


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