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Synthetic peptide corresponding to Human Notch2 aa 2396-2409 conjugated to Keyhole Limpet Haemocyanin (KLH) (Cysteine residue). Synthetic peptide:
NAAERTPSHSGHLQconjugated to KLH with a Cysteine residue linker, corresponding to amino acids 2396-2409 of Human Notch2 (NP_077719.2).
Our Abpromise guarantee covers the use of ab118824 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/400 - 1/2000. Predicted molecular weight: 265 kDa.|
|ELISA||1/30000 - 1/90000.|
|IHC-P||1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent concentration.|
ab118824, at a 1/500 dilution, staining Notch2 in formalin fixed, paraffin embedded Human kidney tissue followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.