Anti-Notch2 antibody (ab118824)
Key features and details
- Rabbit polyclonal to Notch2
- Suitable for: ELISA, ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-Notch2 antibody
See all Notch2 primary antibodies -
Description
Rabbit polyclonal to Notch2 -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human Notch2 aa 2350-2450 conjugated to keyhole limpet haemocyanin (Cysteine residue). Synthetic peptide:
NAAERTPSHSGHLQ
conjugated to KLH with a Cysteine residue linker, corresponding to amino acids 2396-2409 of Human Notch2 (NP_077719.2). -
Positive control
- Human kidney tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 0.424% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading...
-
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab118824 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ELISA |
Use at an assay dependent concentration.
|
|
ICC/IF |
Use at an assay dependent concentration.
|
|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 265 kDa.
|
|
IHC-P |
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
ELISA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 265 kDa. |
IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation. Positively regulates self-renewal of liver cancer cells (PubMed:25985737). -
Tissue specificity
Expressed in the brain, heart, kidney, lung, skeletal muscle and liver. Ubiquitously expressed in the embryo. -
Involvement in disease
Alagille syndrome 2
Hajdu-Cheney syndrome -
Sequence similarities
Belongs to the NOTCH family.
Contains 6 ANK repeats.
Contains 35 EGF-like domains.
Contains 3 LNR (Lin/Notch) repeats. -
Post-translational
modificationsSynthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
Hydroxylated by HIF1AN.
Can be either O-glucosylated or O-xylosylated at Ser-613 by POGLUT1. -
Cellular localization
Cell membrane and Nucleus. Cytoplasm. Following proteolytical processing NICD is translocated to the nucleus. Retained at the cytoplasm by C8orf4 (PubMed:25985737). - Information by UniProt
-
Database links
- Entrez Gene: 4853 Human
- Entrez Gene: 18129 Mouse
- Omim: 600275 Human
- SwissProt: Q04721 Human
- SwissProt: O35516 Mouse
- SwissProt: Q9QW30 Rat
- Unigene: 487360 Human
- Unigene: 485843 Mouse
-
Alternative names
- AGS2 antibody
- hN2 antibody
- Motch B antibody
see all
Images
Datasheets and documents
-
SDS download
-
Datasheet download
References (9)
ab118824 has been referenced in 9 publications.
- Li C et al. MIR4435-2HG in exosomes promotes gastric carcinogenesis by inducing M2 polarization in macrophages. Front Oncol 12:1017745 (2022). PubMed: 36483041
- Yang Z et al. Extracellular vesicle-derived microRNA-18b ameliorates preeclampsia by enhancing trophoblast proliferation and migration via Notch2/TIM3/mTORC1 axis. J Cell Mol Med 25:4583-4595 (2021). PubMed: 33835684
- Yuan Y et al. MicroRNA-16 is involved in the pathogenesis of pre-eclampsia via regulation of Notch2. J Cell Physiol 235:4530-4544 (2020). PubMed: 31643078
- Xue F et al. Neferine inhibits growth and migration of gastrointestinal stromal tumor cell line GIST-T1 by up-regulation of miR-449a. Biomed Pharmacother 109:1951-1959 (2019). PubMed: 30551450
- López-Arribillaga E et al. Manic Fringe deficiency imposes Jagged1 addiction to intestinal tumor cells. Nat Commun 9:2992 (2018). PubMed: 30065304
- Hu X et al. Long non-coding RNA GAS5 aggravates hypoxia injury in PC-12 cells via down-regulating miR-124. J Cell Biochem 119:6765-6774 (2018). PubMed: 29737538
- Hurst LA et al. TNFa drives pulmonary arterial hypertension by suppressing the BMP type-II receptor and altering NOTCH signalling. Nat Commun 8:14079 (2017). IHC . PubMed: 28084316
- Haider S et al. Notch signaling plays a critical role in motility and differentiation of human first-trimester cytotrophoblasts. Endocrinology 155:263-74 (2014). PubMed: 24189144
- Sarode B et al. Notch signaling in the pigmented epithelium of the anterior eye segment promotes ciliary body development at the expense of iris formation. Pigment Cell Melanoma Res 27:580-9 (2014). PubMed: 24628886