• Product nameAnti-Notch2 antibody
    See all Notch2 primary antibodies
  • Description
    Rabbit polyclonal to Notch2
  • Tested applicationsSuitable for: ELISA, ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Notch2 aa 1719-1733 (N terminal) (acetyl C) (Cysteine residue). The epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form.


    (Peptide available as ab1389, ab205702)



Our Abpromise guarantee covers the use of ab8926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/30000 - 1/90000.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 20706108
WB Use at an assay dependent concentration. Can be blocked with Notch2 peptide (ab205702).


  • FunctionFunctions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation. Positively regulates self-renewal of liver cancer cells (PubMed:25985737).
  • Tissue specificityExpressed in the brain, heart, kidney, lung, skeletal muscle and liver. Ubiquitously expressed in the embryo.
  • Involvement in diseaseAlagille syndrome 2
    Hajdu-Cheney syndrome
  • Sequence similaritiesBelongs to the NOTCH family.
    Contains 6 ANK repeats.
    Contains 35 EGF-like domains.
    Contains 3 LNR (Lin/Notch) repeats.
  • Post-translational
    Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
    Hydroxylated by HIF1AN.
    Can be either O-glucosylated or O-xylosylated at Ser-613 by POGLUT1.
  • Cellular localizationCell membrane and Nucleus. Cytoplasm. Following proteolytical processing NICD is translocated to the nucleus. Retained at the cytoplasm by C8orf4 (PubMed:25985737).
  • Information by UniProt
  • Database links
  • Alternative names
    • AGS2 antibody
    • hN2 antibody
    • Motch B antibody
    • N2 antibody
    • N2ECD antibody
    • N2ICD antibody
    • neurogenic locus notch homolog protein 2 antibody
    • NOTC2_HUMAN antibody
    • Notch 2 antibody
    • Notch 2 intracellular domain antibody
    • Notch Drosophila homolog 2 antibody
    • Notch homolog 2 antibody
    • Notch homolog 2 Drosophila antibody
    • Notch2 antibody
    see all

Anti-Notch2 antibody images

  • Staining of Human corneal epithelial cells with anti-Notch2 (ab8926, 1/100). The Notch2 (green staining) is localised in the cytoplasm. The nucleus is stained with Bis benzimide (1/500)

References for Anti-Notch2 antibody (ab8926)

This product has been referenced in:
  • Nishio M  et al. Dysregulated YAP1/TAZ and TGF-ß signaling mediate hepatocarcinogenesis in Mob1a/1b-deficient mice. Proc Natl Acad Sci U S A 113:E71-80 (2016). WB ; Mouse . Read more (PubMed: 26699479) »
  • Murta D  et al. In vivo notch signaling blockade induces abnormal spermatogenesis in the mouse. PLoS One 9:e113365 (2014). IHC ; Mouse . Read more (PubMed: 25412258) »

See all 10 Publications for this product

Product Wall

Unfortunately it is not possible for us to sell the pre-immune serum of our rabbits.
I am sorry that on this occasion I could not be of more help.

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (postnatal day 1 testes)
Specification postnatal day 1 testes
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Qing Wen

Verified customer

Submitted Dec 19 2012

Thank you for your inquiry.

The immunogen we used was CRDASNHKRREPVGQD, corresponding to amino acids 1719-1733 of Human activated Notch2.

The Notch 2 intracellular domain is from amino acids 1697 to 2471 and is 775 amino acids long ...

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Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. As we discussed on the phone, ...

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When I was a postdoc I studied gamma-secretase inhibitors, APP, abeta, Notch etc. so I feel like I can interpret the result. I would have expected the activated Notch2 protein to be ~ 77 kD more or less. 20 uM of most gamma-secretase inhibitors if ap...

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Thank you for your reply. I was a postdoc in Weiming Xia's lab for four years and spent a considerable amount of time conducting research on gamma-secretase so I should be able to help you. The immunogen we used was CRDASNHKRREPVGQD, corresponding ...

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I'm sending you another vial of ab8926 as a replacement. For your record it is order# 48528 and you should receive it later this week or early next. Please do let me know how this batch works out for you. As I mentioned, ab8926 is a Fast Track antibody...

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Thank you very much for your patience. As you know, ab8926 is a Fast Track antibody and thus has not been fully characterized. We have not yet had any feedback regarding how this antibody works in applications other than ELISA, and so your feedback is ...

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All the information we have for our Rabbit polyclonal to Notch 2 (activated) is on the datasheet. It has been tested in ELISA against the immunising peptide but so far has not been further tested for species cross reactivity or suitability for use in o...

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