Anti-NOTCH3 antibody [HMN3-133] (Phycoerythrin) (ab95775)

Overview

  • Product nameAnti-NOTCH3 antibody [HMN3-133] (Phycoerythrin)
    See all NOTCH3 primary antibodies
  • Description
    Armenian Hamster monoclonal [HMN3-133] to NOTCH3 (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applicationsSuitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse Notch 3-Fc fusion protein

  • Positive control
    • Mouse C57BL/6 thymoyctes or transfected cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab95775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.25-0.5µg for 105-8 cells. A final volume of 100 µL is recommended for staining the cell sample.



ab101026-Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionFunctions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.
  • Tissue specificityUbiquitously expressed in fetal and adult tissues.
  • Involvement in diseaseDefects in NOTCH3 are the cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) [MIM:125310]. CADASIL causes a type of stroke and dementia of which key features include recurrent subcortical ischemic events and vascular dementia. The disorder affects relatively young adults of both sexes. Mutations affect highly conserved cysteine residues within epidermal growth factor (EGF)-like repeat domains in the extracellular part of the receptor.
  • Sequence similaritiesBelongs to the NOTCH family.
    Contains 5 ANK repeats.
    Contains 34 EGF-like domains.
    Contains 3 LNR (Lin/Notch) repeats.
  • Post-translational
    modifications
    Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
    Phosphorylated.
  • Cellular localizationCell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CADASIL antibody
    • CASIL antibody
    • NOTC3_HUMAN antibody
    • Notch 3 antibody
    • Notch 3 intracellular domain antibody
    • Notch homolog 3 antibody
    • Notch3 antibody
    see all

Anti-NOTCH3 antibody [HMN3-133] (Phycoerythrin) images

  • Staining of CD4+CD8+ (blue histogram) and CD4-CD8-CD25+CD44- (purple histogram) C57BL/6 thymocytes with 0.5 µg of Armenian Hamster IgG Isotype Control PE (left) or 0.25 µg of ab95775 (right). Total viable cells were used for analysis.
  • ICC/IF image of ab95775 stained mouse epiblast stem cells (EpiSCs). The cells were paraformaldehyde fixed and then permeabilized in 0.1% triton, before blocking in 10% Serum, for 1 hour at +4ºC. Cells were then incubated with this antibody (ab95775, 1/200) for 12 hours at +4ºC. DAPI was used to stain the nuclei (blue).

    See Abreview

References for Anti-NOTCH3 antibody [HMN3-133] (Phycoerythrin) (ab95775)

This product has been referenced in:
  • Sekine C  et al. Differential regulation of splenic CD8- dendritic cells and marginal zone B cells by Notch ligands. Int Immunol 21:295-301 (2009). Read more (PubMed: 19181931) »
  • Moriyama Y  et al. Delta-like 1 is essential for the maintenance of marginal zone B cells in normal mice but not in autoimmune mice. Int Immunol 20:763-73 (2008). Read more (PubMed: 18381350) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry regarding ab95775. ab95775 is anti-NOTCH3 antibody conjugated to Phycoerythrin so the signal is red and the nuclei stained with DAPI (blue). The on-line ICC/IF image is an Abreview image submitted by a customer. I hope this h...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (EpiSCs)
Specification EpiSCs
Fixative Paraformaldehyde
Permeabilization Yes - triton 0.1%
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
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Submitted Nov 09 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"