This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Overview

  • Product nameAnti-NOTCH4 antibody
    See all NOTCH4 primary antibodies
  • Description
    Rabbit polyclonal to NOTCH4

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • SpecificityBy sequence similarity, the Notch intracellular domain (NICD)is believed to be contained within amino acids 1467 to 2003. The peptide used for this antibody is found within this region.
  • Species reactivity

    Predicted to work with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1800 - 1900 of Human NOTCH4.

    (Peptide available as ab26877.)

Properties

Applications

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 210 kDa (predicted molecular weight: 210 kDa).

Target

  • FunctionFunctions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system.
  • Tissue specificityHighly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukocytes.
  • Sequence similaritiesBelongs to the NOTCH family.
    Contains 5 ANK repeats.
    Contains 28 EGF-like domains.
    Contains 3 LNR (Lin/Notch) repeats.
  • Post-translational
    modifications
    Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
    Phosphorylated.
  • Cellular localizationCell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ16302 antibody
    • hNotch 4 antibody
    • hNotch4 antibody
    • INT3 antibody
    • Neurogenic locus notch homolog protein 4 antibody
    • NOTC4_HUMAN antibody
    • Notch 4 antibody
    • Notch 4 intracellular domain antibody
    • Notch homolog 4 antibody
    • NOTCH3 antibody
    • Notch4 antibody
    • NOTCH4 protein antibody
    see all

Anti-NOTCH4 antibody images

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • Anti-NOTCH4 antibody (ab33163) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 210 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab33163 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab33163, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293 cells at 1µg/ml.
  • Anti-NOTCH4 antibody (ab33163) at 1 µg/ml + NOTCH4 overexpression lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 210 kDa
    Observed band size : 210,238 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 52 kDa,60 kDa. We are unsure as to the identity of these extra bands.

References for Anti-NOTCH4 antibody (ab33163)

ab33163 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Kidney, bowman's capsule Cells)
Specification Kidney, bowman's capsule Cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% Triton X 100
Blocking step BSA+FBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
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Submitted Aug 05 2010

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (skeletal muscle)
Specification skeletal muscle
Fixative PFA 1%
Permeabilization Yes - PBS-Triton X-100 0.3%
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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Submitted Jun 11 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"