Recombinant
RabMAb

Anti-NOTCH4 antibody [EPR1246(2)] (ab170951)

Overview

  • Product name
    Anti-NOTCH4 antibody [EPR1246(2)]
    See all NOTCH4 primary antibodies
  • Description
    Rabbit monoclonal [EPR1246(2)] to NOTCH4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NOTCH4 aa 1950 to the C-terminus. The exact sequence is proprietary.
    Database link: Q99466

  • Positive control
    • HACAT, HUVEC, Human fetal heart and Saos-2 lysates.
  • General notes

    A trial size is available to purchase for this antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab170951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 210 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function
      Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system.
    • Tissue specificity
      Highly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukocytes.
    • Sequence similarities
      Belongs to the NOTCH family.
      Contains 5 ANK repeats.
      Contains 28 EGF-like domains.
      Contains 3 LNR (Lin/Notch) repeats.
    • Post-translational
      modifications
      Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
      Phosphorylated.
    • Cellular localization
      Cell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ16302 antibody
      • hNotch 4 antibody
      • hNotch4 antibody
      • INT3 antibody
      • Neurogenic locus notch homolog protein 4 antibody
      • NOTC4_HUMAN antibody
      • Notch 4 antibody
      • Notch 4 intracellular domain antibody
      • Notch homolog 4 antibody
      • NOTCH3 antibody
      • Notch4 antibody
      • NOTCH4 protein antibody
      see all

    Images

    • All lanes : Anti-NOTCH4 antibody [EPR1246(2)] (ab170951) at 1/10000 dilution

      Lane 1 : HACAT cell lysate
      Lane 2 : HUVEC cell lysate
      Lane 3 : Human fetal heart lysate
      Lane 4 : Saos-2 cell lysate

      Predicted band size: 210 kDa

    References

    ab170951 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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