Anti-NOTCH4 antibody [HMN4-14] (Phycoerythrin) (ab95776)

Overview

  • Product nameAnti-NOTCH4 antibody [HMN4-14] (Phycoerythrin)
    See all NOTCH4 primary antibodies
  • Description
    Armenian Hamster monoclonal [HMN4-14] to NOTCH4 (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse NOTCH4-Fc recombinant protein

  • Positive control
    • CD8+CD11c+ C57BL/6 splenocytes

Properties

Applications

Our Abpromise guarantee covers the use of ab95776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 105-8 cells.

Target

  • FunctionFunctions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system.
  • Tissue specificityHighly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukocytes.
  • Sequence similaritiesBelongs to the NOTCH family.
    Contains 5 ANK repeats.
    Contains 28 EGF-like domains.
    Contains 3 LNR (Lin/Notch) repeats.
  • Post-translational
    modifications
    Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
    Phosphorylated.
  • Cellular localizationCell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ16302 antibody
    • hNotch 4 antibody
    • hNotch4 antibody
    • INT3 antibody
    • Neurogenic locus notch homolog protein 4 antibody
    • NOTC4_HUMAN antibody
    • Notch 4 antibody
    • Notch 4 intracellular domain antibody
    • Notch homolog 4 antibody
    • NOTCH3 antibody
    • Notch4 antibody
    • NOTCH4 protein antibody
    see all

Anti-NOTCH4 antibody [HMN4-14] (Phycoerythrin) images

  • Flow cytometric analysis of CD8+CD11c+ C57BL/6 splenocytes with 0.25 µg of Armenian Hamster IgG Isotype Control PE (blue histogram) or ab95776 at 0.5 µg (purple histogram). Total viable cells were used for analysis.

References for Anti-NOTCH4 antibody [HMN4-14] (Phycoerythrin) (ab95776)

ab95776 has not yet been referenced specifically in any publications.

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