Overview

  • Product name
    Anti-Nova2 antibody
    See all Nova2 primary antibodies
  • Description
    Rabbit polyclonal to Nova2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Zebrafish
  • Immunogen

    A region within synthetic peptide: TVSGEPEQVH KAVSAIVQKV QEDPQSSSCL NISYANVAGP VANSNPTGSP, corresponding to internal sequence amino acids 181-230 of Human Nova2

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • HepG2 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab51004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 49 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent dilution.

Target

  • Relevance
    NOVA2 may regulate RNA splicing or metabolism in a specific subset of developing neurons. It binds single strand RNA.
  • Cellular localization
    Nuclear
  • Database links
    • Alternative names
      • ANOVA antibody
      • Astrocytic NOVA1 like RNA binding protein antibody
      • Gm1424 antibody
      • Neuro oncological ventral antigen 2 antibody
      • Nova3 antibody
      • RNA binding protein Nova 2 antibody
      see all

    Images

    • Western blot - Anti-Nova2 antibody (ab51004)
      Western blot - Anti-Nova2 antibody (ab51004)
      Anti-Nova2 antibody (ab51004) at 2.5 µg/ml + HepG2 cell lysate at 10 µg

      Secondary
      HRP conjugated anti-Rabbit Ig at 1/50000 dilution

      Predicted band size: 49 kDa
      Observed band size: 59 kDa (why is the actual band size different from the predicted?)

    References

    ab51004 has not yet been referenced specifically in any publications.

    Publishing research using ab51004? Please let us know so that we can cite the reference in this datasheet.

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