Overview

  • Product name
  • Description
    Rabbit polyclonal to NOX1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human NOX1.

  • Positive control
    • This antibody gave a positive signal in whole cell lysates: DU145; PC3; LNCaP; HepG2; Caco2; HeLa.

Properties

Applications

Our Abpromise guarantee covers the use of ab101027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 75 kDa (predicted molecular weight: 65 kDa).

Target

  • Function
    NOH-1S is a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain.
  • Tissue specificity
    NOH-1L is detected in colon, uterus, prostate, and colon carcinoma, but not in peripheral blood leukocytes. NOH-1S is detected only in colon and colon carcinoma cells.
  • Sequence similarities
    Contains 1 FAD-binding FR-type domain.
    Contains 1 ferric oxidoreductase domain.
  • Cellular localization
    Cell projection > invadopodium membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • GP91 2 antibody
    • Mitogenic oxidase (pyridine nucleotide dependent superoxide generating) antibody
    • Mitogenic oxidase 1 antibody
    • MOX-1 antibody
    • MOX1 antibody
    • NADH/NADPH mitogenic oxidase subunit P65 MOX antibody
    • NADH/NADPH mitogenic oxidase subunit P65-MOX antibody
    • NADPH oxidase 1 antibody
    • NADPH oxidase 1 variant NOH 1L antibody
    • NADPH oxidase homolog 1 antibody
    • NOH 1 antibody
    • NOH-1 antibody
    • NOH1 antibody
    • NOX-1 antibody
    • Nox1 antibody
    • NOX1_HUMAN antibody
    • RP1 146H21.1 antibody
    see all

Images

  • All lanes : Anti-NOX1 antibody (ab101027) at 1/250 dilution

    Lane 1 : DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate
    Lane 2 : PC3 whole cell lysate (ab3954)
    Lane 3 : LNCaP human prostate carcinoma cell line lysate Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 65 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 39 kDa,49 kDa,67 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

References

ab101027 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab101027.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up