Overview

  • Product name
  • Description
    Rabbit polyclonal to NOX1
  • Tested applications
    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human, Pig
  • Immunogen

    Synthetic peptide corresponding to C terminal residues of human NOX1.

Properties

Applications

Our Abpromise guarantee covers the use of ab55831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
ELISA
  • Application notes
    ELISA: Use at an assay dependent dilution.
    ICC/IF: Use at a concentration of 1 µg/ml.
    WB: Use at an assay dependent concentration. Predicted molecular weight: 65 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      NOH-1S is a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain.
    • Tissue specificity
      NOH-1L is detected in colon, uterus, prostate, and colon carcinoma, but not in peripheral blood leukocytes. NOH-1S is detected only in colon and colon carcinoma cells.
    • Sequence similarities
      Contains 1 FAD-binding FR-type domain.
      Contains 1 ferric oxidoreductase domain.
    • Cellular localization
      Cell projection > invadopodium membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • GP91 2 antibody
      • Mitogenic oxidase (pyridine nucleotide dependent superoxide generating) antibody
      • Mitogenic oxidase 1 antibody
      • MOX-1 antibody
      • MOX1 antibody
      • NADH/NADPH mitogenic oxidase subunit P65 MOX antibody
      • NADH/NADPH mitogenic oxidase subunit P65-MOX antibody
      • NADPH oxidase 1 antibody
      • NADPH oxidase 1 variant NOH 1L antibody
      • NADPH oxidase homolog 1 antibody
      • NOH 1 antibody
      • NOH-1 antibody
      • NOH1 antibody
      • NOX-1 antibody
      • Nox1 antibody
      • NOX1_HUMAN antibody
      • RP1 146H21.1 antibody
      see all

    Anti-NOX1 antibody images

    • ICC/IF image of ab55831 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55831, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References for Anti-NOX1 antibody (ab55831)

    This product has been referenced in:
    • Yu P  et al. A dual role of transient receptor potential melastatin 2 channel in cytotoxicity induced by silica nanoparticles. Sci Rep 5:18171 (2015). WB . Read more (PubMed: 26656285) »
    • Van Hée VF  et al. Lactate does not activate NF-?B in oxidative tumor cells. Front Pharmacol 6:228 (2015). WB ; Human . Read more (PubMed: 26528183) »

    See all 10 Publications for this product

    Product Wall

    Application
    Immunoprecipitation
    Immuno-precipitation step
    Other - Gamma-bind plus protein A/G (half/half)
    Sample
    Human Cell lysate - whole cell (KERATINOCYTES, Hacat B)
    Specification
    KERATINOCYTES, Hacat B
    Treatment
    inhibitor of nox 50´M
    Total protein in input
    1000 µg
    Username

    Dr. houssam raad

    Verified customer

    Submitted Mar 12 2015

    Abreviews
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Non-reduced Denaturing (Any Kd)
    Sample
    Rat Tissue lysate - whole (brain)
    Specification
    brain
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 13 2014

    Application
    Western blot
    Loading amount
    40 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Tissue lysate - whole (Liver)
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 06 2014

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Sample
    Human Cell (colon cancer cell)
    Specification
    colon cancer cell
    Permeabilization
    Yes - 0.1% Tween 20
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Feb 28 2014

    I am very pleased to hear you would like to accept our offer and test ab55831 in mouse. This code will give you $347 off your next order before the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the ...

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