Tissue/ cell preparation (Human): Partially Purified human neutrophil flavocytochrome b (heparin Ultrogel/lectin affinity purification in Triton X-100).
Our Abpromise guarantee covers the use of ab80897 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).|
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|