The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 - 4 µg/ml.
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 65.3 kDa).
Use at an assay dependent concentration.
Antibody detection limit dilution 1:2,000.
Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.
Involvement in disease
Defects in CYBB are a cause of chronic granulomatous disease X-linked (XCGD) [MIM:306400]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
Immunohistochemical analysis of paraffin embedded Human Spleen tissue labeling NOX2/gp91phox with ab106940 at 2 ug/ml. Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The HRP-staining procedure was used for detection.
Western blot - Anti-NOX2/gp91phox antibody (ab106940)
Anti-NOX2/gp91phox antibody (ab106940) at 0.03 µg/ml + Peripheral Blood Mononucleocytes lysate at 35 µg