Overview

  • Product name
    Anti-NOX2/gp91phox antibody
    See all NOX2/gp91phox primary antibodies
  • Description
    Goat polyclonal to NOX2/gp91phox
  • Tested applications
    Suitable for: IHC, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide corresponding to Human NOX2/gp91phox aa 151-163 (internal sequence).
    Sequence:

    C-SYLNFARKRIKNP


    Database link: P04839

  • Positive control
    • Human Peripheral Blood Mononucleocytes lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab106940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC Use a concentration of 2 - 4 µg/ml.
WB Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 65.3 kDa).
ELISA Use at an assay dependent concentration.

Antibody detection limit dilution 1:2,000.

Target

  • Function
    Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.
  • Involvement in disease
    Defects in CYBB are a cause of chronic granulomatous disease X-linked (XCGD) [MIM:306400]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
  • Sequence similarities
    Contains 1 FAD-binding FR-type domain.
    Contains 1 ferric oxidoreductase domain.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AMCBX2 antibody
    • CGD antibody
    • CGD91-phox antibody
    • CY24B_HUMAN antibody
    • CYBB antibody
    • Cytochrome b 245, beta polypeptide antibody
    • Cytochrome b(558) beta chain antibody
    • Cytochrome b(558) subunit beta antibody
    • Cytochrome b-245 heavy chain antibody
    • Cytochrome b558 subunit beta antibody
    • GP91 PHOX antibody
    • gp91-1 antibody
    • gp91-phox antibody
    • GP91PHOX antibody
    • Heme-binding membrane glycoprotein gp91phox antibody
    • NADPH oxidase 2 antibody
    • Neutrophil cytochrome b 91 kDa polypeptide antibody
    • NOX2 antibody
    • p22 phagocyte B-cytochrome antibody
    • P91 PHOX antibody
    • p91-PHOX antibody
    • Superoxide-generating NADPH oxidase heavy chain subunit antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded Human Spleen tissue labeling NOX2/gp91phox with ab106940 at 2 ug/ml. Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The HRP-staining procedure was used for detection.

  • Anti-NOX2/gp91phox antibody (ab106940) at 0.03 µg/ml + Peripheral Blood Mononucleocytes lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 65.3 kDa

References

ab106940 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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