Overview

  • Product nameAnti-NOX3 antibody
    See all NOX3 primary antibodies
  • Description
    Rabbit polyclonal to NOX3
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human NOX3.

    (Peptide available as ab101218.)

  • Positive control
    • This antibody gave a positive signal in HeLa and HEK293 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab81864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 64 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

Anti-NOX3 antibody images

  • All lanes : Anti-NOX3 antibody (ab81864) at 1 µg/ml

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 64 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 105 kDa,85 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab81864 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81864, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% MCF7 fixed (10 min) MCF7 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa cells at 1µg/ml

References for Anti-NOX3 antibody (ab81864)

ab81864 has not yet been referenced specifically in any publications.

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