Overview

  • Product name
  • Description
    Rabbit polyclonal to Noxa
  • Specificity
    Although immunogen is from Mouse, customer feedback indicates that the results for Mouse are non-specific (see Abreviews).
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to 17 amino acids at the amino terminus of mouse Noxa. (Peptide available as ab39823.)

  • Positive control
    • Human stomach tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab36833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 11.6 (mouse), 6 (human) kDa.Can be blocked with Human Noxa peptide (ab39823).
IHC-P Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 1 - 5 µg/ml.

Target

  • Function
    Promotes activation of caspases and apoptosis. Promotes mitochondrial membrane changes and efflux of apoptogenic proteins from the mitochondria. Contributes to p53/TP53-dependent apoptosis after radiation exposure. Promotes proteasomal degradation of MCL1. Competes with BAK1 for binding to MCL1 and can displace BAK1 from its binding site on MCL1 (By similarity). Competes with BIM/BCL2L11 for binding to MCL1 and can displace BIM/BCL2L11 from its binding site on MCL1.
  • Tissue specificity
    Highly expressed in adult T-cell leukemia cell line.
  • Sequence similarities
    Belongs to the PMAIP1 family.
  • Domain
    The BH3 motif is essential for pro-apoptotic activity.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adult T cell leukemia derived PMA responsive antibody
    • APR antibody
    • APR_HUMAN antibody
    • ATL-derived antibody
    • Immediate early response protein APR antibody
    • Immediate-early-response protein APR antibody
    • NOXA antibody
    • Phorbol 12 myristate 13 acetate induced protein 1 antibody
    • Phorbol-12-myristate-13-acetate-induced protein 1 antibody
    • PMA induced protein 1 antibody
    • PMA-induced protein 1 antibody
    • PMA-responsive gene antibody
    • Pmaip1 antibody
    • Protein Noxa antibody
    see all

Images

  • Anti-Noxa antibody (ab36833) at 0.5 µg/ml + Human stomach cell lysate

    Predicted band size : 11.6 (mouse), 6 (human) kDa
    Observed band size : 15 kDa (why is the actual band size different from the predicted?)
  • ab36833 at 1µg/ml staining Noxa in human stomach tissue by IHC

  • ICC/IF image of ab36833 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36833, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunofluorescence of Noxa in Human Stomach cells using ab36833 at 10 ug/ml.

References

This product has been referenced in:
  • Liu XJ  et al. Combinatorial effects of miR-20a and miR-29b on neuronal apoptosis induced by spinal cord injury. Int J Clin Exp Pathol 8:3811-8 (2015). Read more (PubMed: 26097563) »
  • Nie P  et al. Synergistic Induction of Erlotinib-Mediated Apoptosis by Resveratrol in Human Non-Small-Cell Lung Cancer Cells by Down-Regulating Survivin and Up-Regulating PUMA. Cell Physiol Biochem 35:2255-71 (2015). Read more (PubMed: 25895606) »

See all 9 Publications for this product

Customer reviews and Q&As

Thank you for contacting us. We do not have any recommendation for further optimization. I would like to ask, how you would like to proceed? Would you like to have same antibody from different lot or want refund of your purchase?

Thank you for your enquiry regarding ab36833 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody. Additionally, thank you for supplying an image, as this has...

Read More

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We are still working on your inquiry, we will get back to you soon.

Many thanks for having patience!

Application
Western blot
Sample
Mouse Recombinant protein (Recombinant)
Loading amount
0.05 µg
Specification
Recombinant
Gel Running Conditions
Reduced Denaturing (10% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 04 2009

Application
Western blot
Sample
Human Cell lysate - whole cell (multiple myeloma (RPMI8226) and T-cell leukemia (J)
Loading amount
30 µg
Specification
multiple myeloma (RPMI8226) and T-cell leukemia (J
Treatment
Velcade for 18 h
Blocking step
Milk as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 5%
Username

Mrs. Isabel Marzo

Verified customer

Submitted Aug 01 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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