Overview

  • Product nameAnti-Noxa antibody
    See all Noxa primary antibodies
  • Description
    Rabbit polyclonal to Noxa
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Macaque Monkey, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 to the C-terminus of Human Noxa.

  • Positive control
    • This antibody gave a positive signal in Jurkat whole cell lysate within WB and in FFPE human breast fibroadenoma tissue sections within IHC.

Properties

Applications

Our Abpromise guarantee covers the use of ab85946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 6 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionPromotes activation of caspases and apoptosis. Promotes mitochondrial membrane changes and efflux of apoptogenic proteins from the mitochondria. Contributes to p53/TP53-dependent apoptosis after radiation exposure. Promotes proteasomal degradation of MCL1. Competes with BAK1 for binding to MCL1 and can displace BAK1 from its binding site on MCL1 (By similarity). Competes with BIM/BCL2L11 for binding to MCL1 and can displace BIM/BCL2L11 from its binding site on MCL1.
  • Tissue specificityHighly expressed in adult T-cell leukemia cell line.
  • Sequence similaritiesBelongs to the PMAIP1 family.
  • DomainThe BH3 motif is essential for pro-apoptotic activity.
  • Cellular localizationMitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adult T cell leukemia derived PMA responsive antibody
    • APR antibody
    • APR_HUMAN antibody
    • ATL-derived antibody
    • Immediate early response protein APR antibody
    • Immediate-early-response protein APR antibody
    • NOXA antibody
    • Phorbol 12 myristate 13 acetate induced protein 1 antibody
    • Phorbol-12-myristate-13-acetate-induced protein 1 antibody
    • PMA induced protein 1 antibody
    • PMA-induced protein 1 antibody
    • PMA-responsive gene antibody
    • Pmaip1 antibody
    • Protein Noxa antibody
    see all

Anti-Noxa antibody images

  • Anti-Noxa antibody (ab85946) at 1 µg/ml + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 6 kDa
    Observed band size : 15 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,36 kDa,74 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
  • IHC image of ab85946 staining in human breast fibroadenoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85946, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Noxa antibody (ab85946)

ab85946 has not yet been referenced specifically in any publications.

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