Overview

  • Product name
  • Description
    Mouse polyclonal to NOXA1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human NOXA1 (NP_006638).

  • Positive control
    • NOXA1 transfected 293T lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab68523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa).

Target

  • Function
    Functions as an activator of NOX1, a superoxide-producing NADPH oxidase. Functions in the production of reactive oxygen species (ROS) which participate in a variety of biological processes including host defense, hormone biosynthesis, oxygen sensing and signal transduction. May also activate CYBB/gp91phox and NOX3.
  • Tissue specificity
    Widely expressed. Detected in pancreas, liver, kidney, spleen, prostate, small intestine and colon.
  • Sequence similarities
    Belongs to the NCF2/NOXA1 family.
    Contains 1 OPR domain.
    Contains 1 SH3 domain.
    Contains 4 TPR repeats.
  • Developmental stage
    Expressed in fetal kidney.
  • Domain
    The SH3 domain mediates interaction with NOXO1 and NCF1 and has autoregulatory function.
    The TPR repeats mediate interaction with RAC1.
  • Post-translational
    modifications
    Interaction with YWHAZ depends on phosphorylation by PKA.
  • Cellular localization
    Cytoplasm. Cell membrane. Translocation to membranes depends on NOXO1 or NCF1 and maybe RAC1.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen NY CO 31 antibody
    • Antigen NY-CO-31 antibody
    • FLJ25475 antibody
    • Inhibitory NADPH oxidase activator 1 antibody
    • MGC131800 antibody
    • NADPH oxidase activator 1 antibody
    • NCF2 like protein antibody
    • NCF2-like protein antibody
    • NOX activator 1 antibody
    • NOXA 1 antibody
    • Noxa1 antibody
    • NOXA1_HUMAN antibody
    • NY CO 31 antibody
    • p51 nox antibody
    • p51-nox antibody
    • p51NOX antibody
    • P67phox like factor antibody
    • P67phox-like factor antibody
    • SDCCAG31 antibody
    • Serologically defined colon cancer antigen 31 antibody
    see all

Images

  • All lanes : Anti-NOXA1 antibody (ab68523) at 1/500 dilution

    Lane 1 : NOXA1 transfected 293T lysate
    Lane 2 : Non-transfected 293T lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 51 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Streeter J  et al. Phosphorylation of Nox1 regulates association with NoxA1 activation domain. Circ Res 115:911-8 (2014). Read more (PubMed: 25228390) »
  • Csányi G  et al. Nox2 B-loop peptide, Nox2ds, specifically inhibits the NADPH oxidase Nox2. Free Radic Biol Med 51:1116-25 (2011). WB ; Human . Read more (PubMed: 21586323) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunoprecipitation
Immuno-precipitation step
Other - Gamma-bind beads plus protein A/G (half/half)
Sample
Human Cell lysate - whole cell (Kratnocytes, Hacat B)
Specification
Kratnocytes, Hacat B
Treatment
inhibitor of nox 50´M for 24h
Total protein in input
1000 µg
Username

Dr. houssam raad

Verified customer

Submitted Mar 12 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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