The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa).
FunctionFunctions as an activator of NOX1, a superoxide-producing NADPH oxidase. Functions in the production of reactive oxygen species (ROS) which participate in a variety of biological processes including host defense, hormone biosynthesis, oxygen sensing and signal transduction. May also activate CYBB/gp91phox and NOX3.
Tissue specificityWidely expressed. Detected in pancreas, liver, kidney, spleen, prostate, small intestine and colon.
Sequence similaritiesBelongs to the NCF2/NOXA1 family. Contains 1 OPR domain. Contains 1 SH3 domain. Contains 4 TPR repeats.
Developmental stageExpressed in fetal kidney.
DomainThe SH3 domain mediates interaction with NOXO1 and NCF1 and has autoregulatory function. The TPR repeats mediate interaction with RAC1.
Post-translational modificationsInteraction with YWHAZ depends on phosphorylation by PKA.
Cellular localizationCytoplasm. Cell membrane. Translocation to membranes depends on NOXO1 or NCF1 and maybe RAC1.
Csányi G et al. Nox2 B-loop peptide, Nox2ds, specifically inhibits the NADPH oxidase Nox2. Free Radic Biol Med51:1116-25 (2011).
Read more (PubMed: 21586323) »
Biasi F et al. Pro-oxidant and proapoptotic effects of cholesterol oxidation products on human colonic epithelial cells: a potential mechanism of inflammatory bowel disease progression. Free Radic Biol Med47:1731-41 (2009).
Read more (PubMed: 19778604) »