Anti-NOXA2/p67phox antibody [EPR5064] (ab109366)

Overview

  • Product nameAnti-NOXA2/p67phox antibody [EPR5064]
    See all NOXA2/p67phox primary antibodies
  • Description
    Rabbit monoclonal [EPR5064] to NOXA2/p67phox
  • Tested applicationsSuitable for: ICC, WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. within Human NOXA2/p67phox aa 200-300 (internal sequence). The exact sequence is proprietary.
    Database link: P19878

  • Positive control
    • Human spleen tissue. THP-1 cell lysate. HL-60 cells treated with dexomethasome, dimethyl sulfoxide, and TPA
  • General notes

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/500 - 1/1000.
WB 1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IP 1/10 - 1/100.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionNCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
    • Involvement in diseaseDefects in NCF2 are a cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 2 (CGD2) [MIM:233710]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
    • Sequence similaritiesBelongs to the NCF2/NOXA1 family.
      Contains 1 OPR domain.
      Contains 2 SH3 domains.
      Contains 3 TPR repeats.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • 67 kDa neutrophil oxidase factor antibody
      • Chronic granulomatous disease autosomal 2 antibody
      • FLJ93058 antibody
      • NADPH oxidase activator 2 antibody
      • NCF-2 antibody
      • Ncf2 antibody
      • NCF2_HUMAN antibody
      • Neutrophil cytosol factor 2 antibody
      • Neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2) antibody
      • Neutrophil NADPH oxidase factor 2 antibody
      • NOXA2 antibody
      • P67 PHOX antibody
      • p67-phox antibody
      • p67phox antibody
      see all

    Anti-NOXA2/p67phox antibody [EPR5064] images

    • All lanes : Anti-NOXA2/p67phox antibody [EPR5064] (ab109366) at 1/1000 dilution

      Lane 1 : HL-60 cells treated with dexomethasome, dimethyl sulfoxide, and TPA
      Lane 2 : THP-1 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size : 60 kDa
    • Immunohistochemical analysis of NOXA2/p67phox in paraffin-embedded Human spleen tissue using ab109366 at 1/500 dilution

    References for Anti-NOXA2/p67phox antibody [EPR5064] (ab109366)

    This product has been referenced in:
    • Wilson C  et al. Contribution of NADPH oxidase to the establishment of hippocampal neuronal polarity in culture. J Cell Sci 128:2989-95 (2015). Mouse, Rat . Read more (PubMed: 26101350) »
    • Yang XY  et al. Inhibition of NADPH Oxidase Mediates Protective Effect of Cardiotonic Pills against Rat Heart Ischemia/Reperfusion Injury. Evid Based Complement Alternat Med 2013:728020 (2013). WB ; Rat . Read more (PubMed: 23840265) »

    See all 2 Publications for this product

    Product Wall

    Application Western blot
    Sample Rat Tissue lysate - whole (Skeletal muscle)
    Gel Running Conditions Reduced Denaturing (8%)
    Loading amount 20 µg
    Specification Skeletal muscle
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 01 2016

    Application Western blot
    Sample Mouse Tissue lysate - whole (Skeletal muscle)
    Gel Running Conditions Reduced Denaturing (8%)
    Loading amount 20 µg
    Specification Skeletal muscle
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 01 2016

    Vielen Dank für Ihre Anfrage. Gegen die genannten NADPH-Oxidase-Komponenten haben wir folgende Antikörper bzw. Proteine in unserem Katalog: Click here (or use the following: http://www.abcam.com/index.html?datasheet=109366). Click here (or use the...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"