Anti-NOXA2/p67phox antibody [EPR5065] (ab109523)

Overview

  • Product nameAnti-NOXA2/p67phox antibody [EPR5065]
    See all NOXA2/p67phox primary antibodies
  • Description
    Rabbit monoclonal [EPR5065] to NOXA2/p67phox
  • Tested applicationsSuitable for: WB, IHC-P, ICC, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human NOXA2/p67phox

  • Positive control
    • THP-1 or HL-60 treated with Dexomethasone and TPA cell lysate Human colon tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab109523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 67 kDa (predicted molecular weight: 60 kDa).
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/500 - 1/1000.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for IP.
  • Target

    • FunctionNCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
    • Involvement in diseaseDefects in NCF2 are a cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 2 (CGD2) [MIM:233710]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
    • Sequence similaritiesBelongs to the NCF2/NOXA1 family.
      Contains 1 OPR domain.
      Contains 2 SH3 domains.
      Contains 3 TPR repeats.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • 67 kDa neutrophil oxidase factor antibody
      • Chronic granulomatous disease autosomal 2 antibody
      • FLJ93058 antibody
      • NADPH oxidase activator 2 antibody
      • NCF-2 antibody
      • Ncf2 antibody
      • NCF2_HUMAN antibody
      • Neutrophil cytosol factor 2 antibody
      • Neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2) antibody
      • Neutrophil NADPH oxidase factor 2 antibody
      • NOXA2 antibody
      • P67 PHOX antibody
      • p67-phox antibody
      • p67phox antibody
      see all

    Anti-NOXA2/p67phox antibody [EPR5065] images

    • All lanes : Anti-NOXA2/p67phox antibody [EPR5065] (ab109523) at 1/10000 dilution

      Lane 1 : THP-1 cell lysate
      Lane 2 : HL-60 treated with Dexomethasone and TPA cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 60 kDa
      Observed band size : 67 kDa (why is the actual band size different from the predicted?)
    • ab109523, at a 1/500 dilution, staining Human NOXA2/p67phox in colon, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
    • Flow cytometry analysis of permeabilized THP-1 cells using ab109523 (red) and a rabbit IgG (negative contol) (green). The dilution is 1:150. The secondary antibody is a goat anti-rabbit IgG FITC.

    References for Anti-NOXA2/p67phox antibody [EPR5065] (ab109523)

    ab109523 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"