Overview

  • Product name
  • Description
    Rabbit polyclonal to NOXO1
  • Specificity
    ab34761 recognises alpha, delta and gamma isoforms of NOXO1.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Guinea pig, Dog, Chimpanzee
  • Immunogen

    Recombinant full length protein corresponding to Human NOXO1 aa 238-253.
    Sequence:

    CASRAYESSRADELSV

  • Positive control
    • Recombinant protein (expressed in human 293 cells) and endogenous (detected in human HT-29 colon carcinoma cells).

Properties

Applications

Our Abpromise guarantee covers the use of ab34761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000 - 1/100000.
WB 1/2000 - 1/8000. Detects a band of approximately 50 kDa (predicted molecular weight: 44 kDa).

Target

  • Function
    Constitutively potentiates the superoxide-generating activity of NOX1 and NOX3 and is required for the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity. Isoform 3 is more potent than isoform 1 in activating NOX3. Together with NOXA1, may also substitute to NCF1/p47phox and NCF2/p67phox in supporting the phagocyte NOX2/gp91phox superoxide-generating activity.
  • Tissue specificity
    Expressed in testis, small and large intestines, liver, kidney and pancreas. Isoform 3 is mainly expressed in colon. Isoform 1 is preferentially expressed in testis.
  • Sequence similarities
    Contains 1 PX (phox homology) domain.
    Contains 2 SH3 domains.
  • Developmental stage
    Isoform 3 is expressed in fetal liver.
  • Domain
    The PX domain mediates lipid-binding, localization to the plasma membrane and is required for NOX1 activation.
    The SH3 domains mediate interaction with CYBA/p22phox. Also mediates intramolecular interaction with the proline-rich region.
  • Cellular localization
    Cell membrane. Isoform 3 associates with the plasma membrane in a lipid-dependent manner.
  • Information by UniProt
  • Database links
  • Alternative names
    • NADPH oxidase organizer 1 antibody
    • NADPH oxidase regulatory protein antibody
    • Nox organizer 1 antibody
    • Nox organizing protein 1 antibody
    • Nox-organizing protein 1 antibody
    • NOXO1 antibody
    • NOXO1_HUMAN antibody
    • P41NOX antibody
    • Regulatory protein P41NOX antibody
    • SH3 and PX domain-containing protein 5 antibody
    • SH3PXD5 antibody
    see all

References for Anti-NOXO1 antibody (ab34761)

ab34761 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunoprecipitation
Immuno-precipitation step
Other - gamma bing plus protein A/G
Sample
Human Cell lysate - whole cell (KERATINOCYTES, Hacat B)
Specification
KERATINOCYTES, Hacat B
Treatment
inhibitor of nox 50´M for 24h
Total protein in input
1000 µg
Username

Dr. houssam raad

Verified customer

Submitted Mar 26 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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