Overview

  • Product name
  • Description
    Goat polyclonal to NOXO1
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    RGCVDSVPHPTTEQ

    , corresponding to C terminal amino acids 363-376 of Human NOXO1

  • Positive control
    • Human kidney tissue
  • General notes


    This antibody is expected to recognise all three reported isoforms.

Properties

Applications

Our Abpromise guarantee covers the use of ab77092 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 - 20 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    Constitutively potentiates the superoxide-generating activity of NOX1 and NOX3 and is required for the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity. Isoform 3 is more potent than isoform 1 in activating NOX3. Together with NOXA1, may also substitute to NCF1/p47phox and NCF2/p67phox in supporting the phagocyte NOX2/gp91phox superoxide-generating activity.
  • Tissue specificity
    Expressed in testis, small and large intestines, liver, kidney and pancreas. Isoform 3 is mainly expressed in colon. Isoform 1 is preferentially expressed in testis.
  • Sequence similarities
    Contains 1 PX (phox homology) domain.
    Contains 2 SH3 domains.
  • Developmental stage
    Isoform 3 is expressed in fetal liver.
  • Domain
    The PX domain mediates lipid-binding, localization to the plasma membrane and is required for NOX1 activation.
    The SH3 domains mediate interaction with CYBA/p22phox. Also mediates intramolecular interaction with the proline-rich region.
  • Cellular localization
    Cell membrane. Isoform 3 associates with the plasma membrane in a lipid-dependent manner.
  • Information by UniProt
  • Database links
  • Alternative names
    • NADPH oxidase organizer 1 antibody
    • NADPH oxidase regulatory protein antibody
    • Nox organizer 1 antibody
    • Nox organizing protein 1 antibody
    • Nox-organizing protein 1 antibody
    • NOXO1 antibody
    • NOXO1_HUMAN antibody
    • P41NOX antibody
    • Regulatory protein P41NOX antibody
    • SH3 and PX domain-containing protein 5 antibody
    • SH3PXD5 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded human kidney labelled with ab77092 at 10 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

References

ab77092 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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