Anti-NPAS2 antibody (ab55833)

Overview

  • Product nameAnti-NPAS2 antibody
    See all NPAS2 primary antibodies
  • Description
    Rabbit polyclonal to NPAS2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide corresponding to N-terminal residues of human NPAS2.

Properties

Applications

Our Abpromise guarantee covers the use of ab55833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 92 kDa.
ELISA Use at an assay dependent concentration.

Target

  • FunctionBMAL1-NPAS2 heterodimers activate E-box element (3'-CACGTG-5') transcription of a number of proteins of the circadian clock. This transcription is inhibited in a feedback loop by PER, and also by CRY proteins.
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Basic helix loop helix PAS protein MOP4 antibody
    • Basic-helix-loop-helix-PAS protein MOP4 antibody
    • bHLHe9 antibody
    • class E basic helix loop helix protein 9 antibody
    • Class E basic helix-loop-helix protein 9 antibody
    • FLJ23138 antibody
    • Member of PAS protein 4 antibody
    • Member of PAS superfamily 4 antibody
    • MGC71151 antibody
    • MOP4 antibody
    • Neuronal PAS domain containing protein 2 antibody
    • Neuronal PAS domain protein 2 antibody
    • Neuronal PAS domain-containing protein 2 antibody
    • Neuronal PAS2 antibody
    • NPAS2 antibody
    • NPAS2_HUMAN antibody
    • PAS domain containing protein 4 antibody
    • PAS domain-containing protein 4 antibody
    • PASD4 antibody
    see all

Anti-NPAS2 antibody images

  • Anti-NPAS2 antibody (ab55833) at 1/500 dilution + Mouse brain whole tissue lysate at 25 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 92 kDa
    Observed band size : 92 kDa


    Exposure time : 20 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-NPAS2 antibody (ab55833)

ab55833 has not yet been referenced specifically in any publications.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (brain)
Loading amount 25 µg
Specification brain
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jul 19 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"