Overview

  • Product nameAnti-Npas4 antibody
    See all Npas4 primary antibodies
  • Description
    Goat polyclonal to Npas4
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide:

    CRFNTSKSLRRQS

    , corresponding to internal sequence amino acids 149-161 of Human Npas4 (NP_849195.2).

  • Positive control
    • Human brain (frontal cortex) lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 87 kDa).

Target

Anti-Npas4 antibody images

  • All lanes : Anti-Npas4 antibody (ab109984) at 1 µg/ml

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 87 kDa
  • Anti-Npas4 antibody (ab109984) at 1 µg/ml + Human frontal cortex lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 87 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 hour

References for Anti-Npas4 antibody (ab109984)

ab109984 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"