Purification notesThis antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22 µm membrane.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Predicted molecular weight: 240 kDa.
Use at an assay dependent dilution.
RelevanceNPAT is a 240kDa protein that associates with and can be phosphorylated by the CDK cyclin E–CDK2. The protein level of NPAT peaks at the G1/S boundary.
Overexpression of NPAT accelerates S-phase entry suggesting that NPAT plays a role in S-phase entry.