Overview

  • Product name
  • Description
    Rabbit polyclonal to NPAT
  • Tested applications
    Suitable for: IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 700-750 of Human NPAT (NP_002510.2).

  • Positive control
    • Human 293T cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab99436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Relevance
      NPAT is a 240kDa protein that associates with and can be phosphorylated by the CDK cyclin E–CDK2. The protein level of NPAT peaks at the G1/S boundary. Overexpression of NPAT accelerates S-phase entry suggesting that NPAT plays a role in S-phase entry.
    • Cellular localization
      Nuclear
    • Database links
    • Alternative names
      • E14 antibody
      • nuclear protein ataxia telangiectasia locus antibody
      • nuclear protein in the AT region antibody

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling NPAT with ab99436 at 1/1000 (1µg/ml). Detection: DAB.
    • Immunoprecipitation : 1 mg of 293T cell lysate immunoprecipitated with ab99436 at 3 µg/mg lysate; 20% of immunoprecipitate loaded in lane

      Western Blot: an antibody that recognizes a downstream epitope of NPAT at 1 µg/ml

      developed using the ECL technique

      Exposure time : 30 seconds

      Predicted band size : 154 kDa

    References

    ab99436 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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