The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at 2-5 µg/mg of lysate.
Application notesIs unsuitable for WB.
RelevanceNPAT is a 240kDa protein that associates with and can be phosphorylated by the CDK cyclin E–CDK2. The protein level of NPAT peaks at the G1/S boundary.
Overexpression of NPAT accelerates S-phase entry suggesting that NPAT plays a role in S-phase entry.