Anti-NPEPPS antibody (ab77643)
Key features and details
- Goat polyclonal to NPEPPS
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NPEPPS antibody
See all NPEPPS primary antibodies -
Description
Goat polyclonal to NPEPPS -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Xenopus laevis, Chimpanzee, Macaque monkey -
Immunogen
Synthetic peptide:
C-KRDAESIHQYLLQRK
, corresponding to C terminal amino acids 899-913 of Human NPEPPS (NP_006301.3) -
Positive control
- Human Brain (Cerebral Cortex) lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77643 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Notes |
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WB: Use at a concentration of 0.2 - 0.5 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 103 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Aminopeptidase with broad substrate specificity for several peptides. Involved in proteolytic events essential for cell growth and viability. May act as regulator of neuropeptide activity. Plays a role in the antigen-processing pathway for MHC class I molecules. Involved in the N-terminal trimming of cytotoxic T-cell epitope precursors. Digests the poly-Q peptides found in many cellular proteins. Digests tau from normal brain more efficiently than tau from Alzheimer disease brain. -
Tissue specificity
Detected in liver, epithelium of renal tubules, epithelium of small and large intestine, gastric epithelial cells, and alveoli of the lung (at protein level). -
Sequence similarities
Belongs to the peptidase M1 family. -
Cellular localization
Cytoplasm > cytosol. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9520 Human
- Entrez Gene: 19155 Mouse
- Entrez Gene: 50558 Rat
- Omim: 606793 Human
- SwissProt: P55786 Human
- SwissProt: Q11011 Mouse
- Unigene: 443837 Human
- Unigene: 29824 Mouse
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Alternative names
- AAP-S antibody
- Aminopeptidase puromycin sensitive antibody
- Cytosol alanyl aminopeptidase antibody
see all
Images
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Anti-NPEPPS antibody (ab77643) at 0.3 µg/ml + Human cerebral cortex lysate in RIPA buffer at 35 µg
Predicted band size: 103 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (0)
ab77643 has not yet been referenced specifically in any publications.