The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notespeptide ELISA: Use at an assay dependent dilution. Detection limit dilution: 1/4000
WB: Use at a concentration of 0.2 - 0.5 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 103 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionAminopeptidase with broad substrate specificity for several peptides. Involved in proteolytic events essential for cell growth and viability. May act as regulator of neuropeptide activity. Plays a role in the antigen-processing pathway for MHC class I molecules. Involved in the N-terminal trimming of cytotoxic T-cell epitope precursors. Digests the poly-Q peptides found in many cellular proteins. Digests tau from normal brain more efficiently than tau from Alzheimer disease brain.
Tissue specificityDetected in liver, epithelium of renal tubules, epithelium of small and large intestine, gastric epithelial cells, and alveoli of the lung (at protein level).
Sequence similaritiesBelongs to the peptidase M1 family.