Overview

  • Product name
  • Description
    Goat polyclonal to NPHS2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human, Pig
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to Human NPHS2 aa 365-377 (C terminal) (Cysteine residue).
    Sequence:

    SPSKPVEPLNPKK


    Database link: NP_055440.1

  • Positive control
    • WB: Mouse, human and pig kidney lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab177242 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab177242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa).

Target

  • Function
    Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.
  • Tissue specificity
    Almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli.
  • Involvement in disease
    Defects in NPHS2 are the cause of nephrotic syndrome type 2 (NPHS2) [MIM:600995]. It is a renal disorder characterized clinically by childhood onset of proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. The disorder is resistant to steroid treatment and progresses to end-stage renal failure in the first or second decades. Some patients show later onset of the disorder.
  • Sequence similarities
    Belongs to the band 7/mec-2 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Nephrosis 2, idiopathic, steroid resistant (podocin) antibody
    • nephrosis 2, idiopathic, steroid resistant antibody
    • NPHS2 antibody
    • NPHS2 gene antibody
    • PDCN antibody
    • PODO_HUMAN antibody
    • Podocin antibody
    • SRN1 antibody
    see all

Images

  • All lanes : Anti-NPHS2 antibody (ab177242) at 0.3 µg/ml

    Lane 1 : Human kidney lysate (in RIPA buffer)
    Lane 2 : Mouse kidney lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique

    Predicted band size : 42 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)

    Primary incubation was 1 hour.

  • Anti-NPHS2 antibody (ab177242) at 1 µg/ml + Pig kidney lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 42 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)

    Primary incubation was 1 hour.

References

ab177242 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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