Overview

  • Product nameAnti-NPHS2 antibody
    See all NPHS2 primary antibodies
  • Description
    Rabbit polyclonal to NPHS2
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human NPHS2 (C terminal).
    Database link: NM_014625

  • Positive control
    • Fetal kidney lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human normal kidney.

Properties

Applications

Our Abpromise guarantee covers the use of ab93650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/200 - 1/500. Predicted molecular weight: 42 kDa.

Target

  • FunctionPlays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.
  • Tissue specificityAlmost exclusively expressed in the podocytes of fetal and mature kidney glomeruli.
  • Involvement in diseaseDefects in NPHS2 are the cause of nephrotic syndrome type 2 (NPHS2) [MIM:600995]. It is a renal disorder characterized clinically by childhood onset of proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. The disorder is resistant to steroid treatment and progresses to end-stage renal failure in the first or second decades. Some patients show later onset of the disorder.
  • Sequence similaritiesBelongs to the band 7/mec-2 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Nephrosis 2, idiopathic, steroid resistant (podocin) antibody
    • nephrosis 2, idiopathic, steroid resistant antibody
    • NPHS2 antibody
    • NPHS2 gene antibody
    • PDCN antibody
    • PODO_HUMAN antibody
    • Podocin antibody
    • SRN1 antibody
    see all

Anti-NPHS2 antibody images

  • IHC image of NPHS2 staining in THuman normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93650, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-NPHS2 antibody (ab93650) at 1/200 dilution + Fetal kidney lysate

    Predicted band size : 42 kDa

References for Anti-NPHS2 antibody (ab93650)

This product has been referenced in:
  • Wei MG  et al. JiaWeiDangGui Decoction Ameliorates Proteinuria and Kidney Injury in Adriamycin-Induced Rat by Blockade of TGF-ß/Smad Signaling. Evid Based Complement Alternat Med 2016:5031890 (2016). IHC-P ; Rat . Read more (PubMed: 27403197) »
  • Jeruschke S  et al. Everolimus Stabilizes Podocyte Microtubules via Enhancing TUBB2B and DCDC2 Expression. PLoS One 10:e0137043 (2015). IHC-P . Read more (PubMed: 26331477) »

See all 4 Publications for this product

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"