The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.
Almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli.
Involvement in disease
Defects in NPHS2 are the cause of nephrotic syndrome type 2 (NPHS2) [MIM:600995]. It is a renal disorder characterized clinically by childhood onset of proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. The disorder is resistant to steroid treatment and progresses to end-stage renal failure in the first or second decades. Some patients show later onset of the disorder.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NPHS2 with ab183703 at 1/500 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Western blot - Anti-NPHS2 [EPR13819] antibody (ab183703)
Anti-NPHS2 antibody [EPR13819] (ab183703) at 1/1000 dilution + Human fetal kidney lysate at 10 µg
Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution