Overview

  • Product name
    Anti-NPHS2 antibody [EPR13820]
    See all NPHS2 primary antibodies
  • Description
    Rabbit monoclonal [EPR13820] to NPHS2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NPHS2 aa 350 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9NP85

  • Positive control
    • Human fetal kidney lysate; Human kidney tissue, human skeletal muscle tissue. Rat and mouse kidney.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocol.

WB 1/2000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).

For unpurified use at 1/10000 - 1/50000.

  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.
    • Tissue specificity
      Almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli.
    • Involvement in disease
      Defects in NPHS2 are the cause of nephrotic syndrome type 2 (NPHS2) [MIM:600995]. It is a renal disorder characterized clinically by childhood onset of proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. The disorder is resistant to steroid treatment and progresses to end-stage renal failure in the first or second decades. Some patients show later onset of the disorder.
    • Sequence similarities
      Belongs to the band 7/mec-2 family.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Nephrosis 2, idiopathic, steroid resistant (podocin) antibody
      • nephrosis 2, idiopathic, steroid resistant antibody
      • NPHS2 antibody
      • NPHS2 gene antibody
      • PDCN antibody
      • PODO_HUMAN antibody
      • Podocin antibody
      • SRN1 antibody
      see all

    Images

    • Anti-NPHS2 antibody [EPR13820] (ab181143) at 1/10000 dilution + Human fetal kidney at 20 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 42 kDa

    • Anti-NPHS2 antibody [EPR13820] (ab181143) at 1/50000 dilution (unpurified) + Human fetal kidney lysate at 20 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

      Predicted band size: 42 kDa
      Observed band size: 42 kDa

    • All lanes : Anti-NPHS2 antibody [EPR13820] (ab181143) at 1/2000 dilution

      Lane 1 : Mouse kidney
      Lane 2 : Rat kidney

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 42 kDa
      Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.

    • ab181143 staining NPHS2 in human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

      Negative control 1: PBS in place of primary antibody.

    • ab181143 staining NPHS2 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

      Negative control 1: PBS in place of primary antibody.

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NPHS2 with unpurified ab181143 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    References

    This product has been referenced in:
    • Yu J  et al. Advanced glycation end products induce the apoptosis of and inflammation in mouse podocytes through CXCL9-mediated JAK2/STAT3 pathway activation. Int J Mol Med 40:1185-1193 (2017). Read more (PubMed: 28849106) »

    See 1 Publication for this product

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