• Product nameAnti-NPL4 antibody
    See all NPL4 primary antibodies
  • Description
    Rabbit polyclonal to NPL4
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Rat NPL4.

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HepG2; Caco2; SHSY-5Y; PC3. ICC/IF: HeLa cells.



Our Abpromise guarantee covers the use of ab101226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).


  • FunctionThe ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope.
  • Tissue specificityExpressed at highest levels in brain, heart, skeletal muscle, kidney and fetal liver.
  • PathwayProtein degradation; proteasomal ubiquitin-dependent pathway.
  • Sequence similaritiesBelongs to the NPL4 family.
    Contains 1 RanBP2-type zinc finger.
  • DomainBinds ubiquitinated proteins via its RanBP2-type zinc finger.
  • Cellular localizationCytoplasm > cytosol. Endoplasmic reticulum. Nucleus. Associated with the endoplasmic reticulum and nuclear.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA1499 antibody
    • NPL4 antibody
    • NPL4, S. cerevisiae, homolog of antibody
    • NPL4_HUMAN antibody
    • nploc4 antibody
    • nuclear protein localization 4 homolog (S. cerevisiae) antibody
    • Nuclear protein localization 4 homolog antibody
    • Nuclear protein localization protein 4 homolog antibody
    • Nuclear protein localization protein 4, S. cerevisiae, homolog of antibody
    • Protein NPL4 antibody
    see all

Anti-NPL4 antibody images

  • All lanes : Anti-NPL4 antibody (ab101226) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 4 : PC3 whole cell lysate (ab3954)

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 68 kDa
    Observed band size : 68 kDa

    Exposure time : 8 minutes
  • ICC/IF image of ab101226 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab101226 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in formaldehyde fixed (4%, 10min) Hek293, HepG2, amd MCF-7 cells at 5ug/ml.

References for Anti-NPL4 antibody (ab101226)

ab101226 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TritonX100 in PBS

Dr. Kirk Mcmanus

Verified customer

Submitted Aug 15 2011