Anti-NPL4 antibody (ab68020)
Key features and details
- Mouse polyclonal to NPL4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-NPL4 antibody
See all NPL4 primary antibodies -
Description
Mouse polyclonal to NPL4 -
Host species
Mouse -
Specificity
This antibody is reactive against mammalian transfected lysate. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human NPL4. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
-
Positive control
- Transfected 293T cell lysate and human spleen tissue lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab68020 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/1000. Detects a band of approximately 68, 75 kDa (predicted molecular weight: 68 kDa).
|
Notes |
---|
WB
1/500 - 1/1000. Detects a band of approximately 68, 75 kDa (predicted molecular weight: 68 kDa). |
Target
-
Function
The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. -
Tissue specificity
Expressed at highest levels in brain, heart, skeletal muscle, kidney and fetal liver. -
Pathway
Protein degradation; proteasomal ubiquitin-dependent pathway. -
Sequence similarities
Belongs to the NPL4 family.
Contains 1 RanBP2-type zinc finger. -
Domain
Binds ubiquitinated proteins via its RanBP2-type zinc finger. -
Cellular localization
Cytoplasm > cytosol. Endoplasmic reticulum. Nucleus. Associated with the endoplasmic reticulum and nuclear. - Information by UniProt
-
Database links
- Entrez Gene: 55666 Human
- Omim: 606590 Human
- SwissProt: Q8TAT6 Human
- Unigene: 464333 Human
-
Alternative names
- KIAA1499 antibody
- NPL4 antibody
- NPL4, S. cerevisiae, homolog of antibody
see all
Images
-
Anti-NPL4 antibody (ab68020) at 1/500 dilution + human spleen tissue lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 68 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted? -
All lanes : Anti-NPL4 antibody (ab68020) at 1/500 dilution
Lane 1 : NPLOC4 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa
Datasheets and documents
-
Datasheet download
References (0)
ab68020 has not yet been referenced specifically in any publications.