The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa.
The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope.
Expressed at highest levels in brain, heart, skeletal muscle, kidney and fetal liver.
Protein degradation; proteasomal ubiquitin-dependent pathway.
Belongs to the NPL4 family. Contains 1 RanBP2-type zinc finger.
Binds ubiquitinated proteins via its RanBP2-type zinc finger.
Cytoplasm > cytosol. Endoplasmic reticulum. Nucleus. Associated with the endoplasmic reticulum and nuclear.