Overview

  • Product nameAnti-NPM2 antibody
    See all NPM2 primary antibodies
  • Description
    Rabbit polyclonal to NPM2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cat
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 36-85 (LEGKQSCRLL LHTICLGEKA KEEMHRVEIL PPANQEDKKM QPVTIASLQA) of human NPM2 (NP_877724).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified by peptide affinity chromatography method.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 24 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:312500.

Target

  • FunctionCore histones chaperone involved in chromatin reprogramming, specially during fertilization and early embryonic development. Probably involved in sperm DNA decondensation during fertilization.
  • Sequence similaritiesBelongs to the nucleoplasmin family.
  • DomainThe acidic tract A2 mediates histone binding.
  • Cellular localizationNucleus. Found in the oocyte nucleus before nuclear membrane breakdown, after which it is redistributed to the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • NPM2 antibody
    • NPM2_HUMAN antibody
    • nucleophosmin/nucleoplasmin, 2 antibody
    • nucleoplasmin 2 antibody
    • Nucleoplasmin-2 antibody
    see all

Anti-NPM2 antibody images

  • Anti-NPM2 antibody (ab83310) at 1 µg/ml + placenta lysate at 10 µg with in 5% skim milk / PBS buffer

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 24 kDa
    Observed band size : 24 kDa

References for Anti-NPM2 antibody (ab83310)

ab83310 has not yet been referenced specifically in any publications.

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