Anti-NPTN antibody (ab83063)

Overview

  • Product name
  • Description
    Rabbit polyclonal to NPTN
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 108-157 (SAPKANATIE VKAAPDITGH KRSENKNEGQ DATMYCKSVG YPHPDWIWRK) of Human NPTN (NP_059429).

  • Positive control
    • Fetal muscle lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 32 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Probable homophilic and heterophilic cell adhesion molecule involved in long term potentiation at hippocampal excitatory synapses through activation of p38MAPK. May also regulate neurite outgrowth by activating the FGFR1 signaling pathway. May play a role in synaptic plasticity.
  • Tissue specificity
    Isoform 1 is ubiquitously expressed. Isoform 2 is expressed in brain cortex and cerebellum (at protein level).
  • Sequence similarities
    Contains 3 Ig-like (immunoglobulin-like) domains.
  • Domain
    Some isoforms lack the first Ig-like domain which may confer homophilic adhesion activity. However, they can bind and activate FGFR1.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Cell membrane. Enriched at post-synaptic density.
  • Information by UniProt
  • Database links
  • Alternative names
    • GP55 antibody
    • GP65 antibody
    • Neuroplastin antibody
    • np55 antibody
    • np65 antibody
    • Nptn antibody
    • NPTN_HUMAN antibody
    • SDFR1 antibody
    • SDR-1 antibody
    • SDR1 antibody
    • stromal cell derived factor receptor 1 antibody
    • Stromal cell-derived receptor 1 antibody
    see all

Images

  • Anti-NPTN antibody (ab83063) at 1 µg/ml (5% skim milk / PBS buffer) + Fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 32 kDa
    Observed band size : 31 kDa (why is the actual band size different from the predicted?)

References

ab83063 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us about the issues you are having with the FGFR1 and NPTN antibodies. Can you please send me a few details of your samples and the protocols you use for harvesting the samples and for blotting? In particular, I am interested i...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain Tissue)
Loading amount
40 µg
Specification
Brain Tissue
Gel Running Conditions
Non-reduced Denaturing (10% Bis-Tris)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Verified customer

Submitted May 27 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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