Overview

  • Product name
  • Description
    Rabbit polyclonal to Nptx1
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Rat Nptx1 aa 174-203 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.

  • Positive control
    • Jurkat cell line lysates, human breast carcinoma tissue and human brain tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-P
  • Application notes
    ELISA: 1/1000.
    IHC-P: 1/50 - 1/100.
    WB: 1/50 - 1/100. Detects bands of approximately 47 and 60 kDa (predicted molecular weight: 47 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Images

    • Anti-Nptx1 antibody (ab75917) at 1/60 dilution + Jurkat cell line lysates at 35 µg

      Predicted band size : 47 kDa
      Observed band size : 47 kDa
      Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
    • Formalin-fixed and paraffin-embedded human breast carcinoma reacted with ab75917 at a 1/50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
    • Formalin-fixed and paraffin-embedded human brain tissue reacted with ab75917 at a 1/50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

    References

    ab75917 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab75917.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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