Overview

  • Product nameAnti-NPY1R antibody
    See all NPY1R primary antibodies
  • Description
    Sheep polyclonal to NPY1R
  • Specificityab35336 recognises Neuropeptide-Y Receptor 1.
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide: K-Q-A-S-P-V-A-F-K-K-I-N-N-N conjugated to KLH, corresponding to amino acids 365-378 of Human NPY1R

Properties

Applications

Our Abpromise guarantee covers the use of ab35336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/110.
WB Use at an assay dependent dilution. Predicted molecular weight: 44 kDa.
IHC-P Use at an assay dependent dilution.

Target

References for Anti-NPY1R antibody (ab35336)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (rat skin (free floating 50 µm))
Specification rat skin (free floating 50 µm)
Fixative Paraformaldehyde
Permeabilization Yes - Triton X-100, 0.2%
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jun 25 2012

Thank you for your phone call.
I am sorry to hear that the antibody is not working in frozen sections, but unfortunately we have not tried this application and thus do not know for sure if the antibody would work or not in IHC-Fr.

In ord...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (brain cortex)
Loading amount 100 µg
Specification brain cortex
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 8%
Username

Dr. paolo mele

Verified customer

Submitted Feb 13 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"