Overview

  • Product nameAnti-NPY2R antibody
    See all NPY2R primary antibodies
  • Description
    Rabbit polyclonal to NPY2R
  • Tested applicationsSuitable for: ICC, WB, IHC-P, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    C-TDSFSEATNV

    conjugated to KLH, corresponding to C terminal amino acids 376-385 of NPY2R.

  • General notes


    This antibody has been extensively characterized at the spinal level and is currently being characerized in brain.

Properties

Applications

Our Abpromise guarantee covers the use of ab31894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration. PubMed: 21823118
WB Use at an assay dependent concentration. PubMed: 21823118
IHC-P Use at an assay dependent concentration. PubMed: 21823118
IHC-FoFr 1/500 - 1/1000. Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium free Tyrode's solution, 2) paraformaldehyde picric acid fixative, and 3) 10% sucrose in PBS as a cryo protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS, slide mounted and processed for indirect immunofluorescence.

Target

  • FunctionReceptor for neuropeptide Y and peptide YY. The rank order of affinity of this receptor for pancreatic polypeptides is PYY > NPY > PYY (3-36) > NPY (2-36) > [Ile-31, Gln-34] PP > [Leu-31, Pro-34] NPY > PP, [Pro-34] PYY and NPY free acid.
  • Tissue specificityHigh levels in amygdala, corpus callosum, hippocampus and subthalamic nucleus. Also detectable in caudate nucleus, hypothalamus and substantia nigra.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Neuropeptide Y receptor type 2 antibody
    • Neuropeptide Y receptor Y2 antibody
    • NPY-Y2 receptor antibody
    • NPY2-R antibody
    • NPY2R antibody
    • NPY2R_HUMAN antibody
    • Y2 receptor antibody
    see all

Anti-NPY2R antibody images

  • Ab31894 staining NPY2R in rat hippocampus by immunohistochemistry using perfusion fixed tissue.

References for Anti-NPY2R antibody (ab31894)

This product has been referenced in:
  • Xu X  et al. Altered expression of neuropeptide Y, Y1 and Y2 receptors, but not Y5 receptor, within hippocampus and temporal lobe cortex of tremor rats. Neuropeptides 48:97-105 (2014). IHC-P ; Mouse . Read more (PubMed: 24444822) »
  • Medeiros PJ  et al. Neuropeptide Y stimulates proliferation and migration in the 4T1 breast cancer cell line. Int J Cancer : (2011). WB, IHC-P, ICC ; Mouse . Read more (PubMed: 21823118) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Mouse Cultured Cells (4T1 mammary carcinoma)
Specification 4T1 mammary carcinoma
Fixative Methanol
Permeabilization Yes - 0.25% Triton X100 in PBS
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 1.5% · Temperature: 23°C
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Submitted Oct 18 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"