Overview

  • Product name
  • Description
    Goat polyclonal to NPY5R
  • Specificity
    Ab32886 recognises NPY5R.
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide:

    C-GLSNKENRLEEN

    , corresponding to amino acids 251-262 of Human NPY5R.

  • Positive control
    • Human brain, breast and duodenum lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab32886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/32000.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).

Target

Images

  • Anti-NPY5R antibody (ab32886) at 0.3 µg/ml + Human Brain lysate (35µg protein in RIPA buffer)

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • ab32886 (3.8µg/ml) staining of paraffin embedded Human Intestine shows staining of ganglion cells. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

References

This product has been referenced in:
  • Sheriff S  et al. Neuropeptide Y Y5 receptor promotes cell growth through extracellular signal-regulated kinase signaling and cyclic AMP inhibition in a human breast cancer cell line. Mol Cancer Res 8:604-14 (2010). Read more (PubMed: 20332211) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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