The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).
FunctionReceptor for neuropeptide Y and peptide YY. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. Seems to be associated with food intake. Could be involved in feeding disorders.
Tissue specificityBrain; hypothalamus.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
ab32886 (3.8µg/ml) staining of paraffin embedded Human Intestine shows staining of ganglion cells. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
References for Anti-NPY5R antibody (ab32886)
This product has been referenced in:
Sheriff S et al. Neuropeptide Y Y5 receptor promotes cell growth through extracellular signal-regulated kinase signaling and cyclic AMP inhibition in a human breast cancer cell line. Mol Cancer Res8:604-14 (2010).
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